Dataset Information

Plasma from Rainbow trout housed for 7 days in normoxic and hypoxic water

ABSTRACT: Hypoxic water had 5 mg/L of Dissolved Oxygen (DO) and normoxic water had 11.5 mg/L DO. 40 immature rainbow trout (about 100 g) were housed for 7 days under these conditions (20 fish in each treatment). Then fish were anesthetized with 100 mg/L MS-222, and blood was sampled using caudal puncture with heparinized syringes. The blood was centrifuged at 10 000 x g for 4 minutes in Heraeus Fresco 21 centrifuge (Thermo Fisher). Plasma was removed from this using a pipette, and placed in cryotubes, flash frozen in liquid nitrogen, and then stored at -80oC until preparation for proteomic analysis.

INSTRUMENT(S): 6530A Q-TOF LC/MS

ORGANISM(S): Oncorhynchus Mykiss (rainbow Trout) (salmo Gairdneri)

TISSUE(S): Blood Plasma

SUBMITTER: Denina Simmons

LAB HEAD: Dr, Denina Simmons

PROVIDER: PXD026589 | Pride | 2021-06-18

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	Full_set_after_skyline_with_no_enzyme.xlsx	Xlsx
	checksum.txt	Txt
	raw_files_hypoxia.zip	Other
	raw_files_normoxia.zip	Other

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Publications

Hypoxic responses in Oncorhynchus mykiss involve angiogenesis, lipid, and lactate metabolism, which may be triggered by the cortisol stress response and epigenetic methylation.

Léger Jessica A D JAD Athanasio Camila G CG Zhera Aaleen A Chauhan Mohammed Faiz MF Simmons Denina B D DBD

Comparative biochemistry and physiology. Part D, Genomics & proteomics 20210611

The incidence of hypoxia in water bodies is increasing more rapidly than aquatic life can adapt. This study aimed to determine the effects of hypoxia on fish physiology, as well as protein expression through proteomics. To do this, 40 rainbow trout were divided into normoxic control (11.5 mg/L dissolved oxygen) and hypoxic treatment (5 mg/L dissolved oxygen) tanks for a period of 7 days. Fish were then anesthetized and blood was sampled. Fish were then euthanized and heart and liver samples were ...[more]

PMID: 34126312

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