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Stem cells from human exfoliated deciduous teeth cultured with human platelet lysate

ABSTRACT: Stem cells from human exfoliated deciduous teeth (SHED) can be used as a potential clinical material. But the use of xenogeneic ingredients, especially fetal bovine serum, will increase the risk of zoonotic disease transmission during the use of animal-derived products. Human-platelet-lysate (hPL) was used in human cell expansion with reliability in clinical applications. This study aimed to explore the feasibility and effect of HPL in the cultivation of SHED. We use proteomics to interpret the impact of HPL on the protein profile of SHED. Propagation, differentiation, qPCR and immunofluorescence were used to detect the effect of HPL on SHED.

INSTRUMENT(S): LTQ Orbitrap

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Stem Cell

DISEASE(S): Disease Free

SUBMITTER: Tong Lei

LAB HEAD: Hongwu Du

PROVIDER: PXD026687 | Pride | 2022-05-19

REPOSITORIES: Pride

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			Action	DRS
	HPL1.raw	Raw
	HPL2.raw	Raw
	HPL3.raw	Raw
	SHED_HPL.txt	Txt

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Publications

Hydrogel supplemented with human platelet lysate enhances multi-lineage differentiation of mesenchymal stem cells.

Lei Tong T Liu Yanyan Y Deng Shiwen S Xiao Zhuangzhuang Z Yang Yanjie Y Zhang Xiaoshuang X Bi Wangyu W Du Hongwu H

Journal of nanobiotechnology 20220402 1

Stem cells from human exfoliated deciduous teeth (SHED) can be used as a potential clinical material. But the use of xenogeneic ingredients will increase the risk of zoonotic disease transmission. Human platelet lysate (HPL) is a potential surrogate and used in human cell expansion with reliability in clinical applications. In this study, we synthesized chitosan/gelatin/gellan gum hydrogel supplemented with HPL and investigated the effect of 3D culture for SHED. TMT-tagged proteomics was used to ...[more]

PMID: 35366889

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