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A novel method of spontaneously-released extracellular vesicle isolation from brain

ABSTRACT: Extracellular Vesicles (EVs) exhibit great potential in for brain disease diagnositics and treatment, representing a valuable tool in the new era of pPrecision medicine which demands high-quality human biospecimens. However, current brain exosome EV isolation approaches rely on its tissue dissociation, which may can contaminate the exosome EV fractions with immature intralumenalcellular vesicles that wcould otherwise be targeted for endolysosomal degradation. Hereby, we present an efficient exosome purification method that captures a more physiologically relevant EV population exosome-enriched extracellular vesicles (EVs) spontaneously released by mouse and human brain tissue, representing a more physiologically relevant exosome EV population.

INSTRUMENT(S): LTQ Orbitrap

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Cerebellar Cortex

SUBMITTER: Martina Samiotaki

LAB HEAD: Ioannis Sotiropoulos

PROVIDER: PXD026712 | Pride | 2023-08-03

REPOSITORIES: Pride

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			Action	DRS
	Exosome_LFQ_results.txt	Txt
	Sample_1_exo.raw	Raw
	Sample_1_prot.raw	Raw
	Sample_2_exo.raw	Raw
	Sample_2_prot.raw	Raw

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Project description:Extracellular vesicles (EVs), including small EVs (sEVs) such as exosomes, exhibit great potential for the diagnosis and treatment of brain disorders, representing an advantageous tool for Precision medicine. The latter demands high-quality human biospecimens, especially in complex disorders in which pathological and specimen heterogeneity as well diverse individual clinical profile often complicate the development of precision therapeutic schemes and patient-tailored treatments. Thus, the collection and characterization of physiologically relevant sEVs are of the utmost importance. However, standard brain EV isolation approaches rely on tissue dissociation, which can contaminate EV fractions with intracellular vesicles. Based on multiscale analytical platforms such as cryo-EM, label-free proteomics, advanced flow cytometry, and ExoView analyses, we hereby present an efficient purification method that captures a more physiologically relevant, small EV-enriched population spontaneously released by mouse and human brain tissue. The spontaneous release method of sEV yield may contribute to the characterization and biomarker profile of physiologically relevant brain-derived sEVs in brain function and pathology.

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