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Profiling of glioma C6 and astrocytes rat cell lines before and after co-cultivation.

ABSTRACT: We have grown C6 glioma cells and rat astrocytes, as well as astrocyte cells co-cultured together with C6 glioma. We performed proteome-wide LC-MS analysis of this experimental groups. The data including LC-MS/MS raw files and exported MaxQuant report. For our co-cultivated in vitro model we used astrocytes and C6 glioma cells. Astrocytes cell lines isolated from rat brain tissue. We analyzed astrocytes in two conditions: beforeand after co-cultivation. Proteins were assessed in an untargeted label-free bottom-up proteomic experiment using IDA approach (i.e. InformationDependent Acquisition) on AB Sciex TripleTOF 6600 Q-TOF mass-spectrometer coupled with LFQ (label-free quantification) approach by MaxQuant software. Dataset covers 165 samples (11 biological rand 5 technical replicates)

INSTRUMENT(S): TripleTOF 6600

ORGANISM(S): Rattus Norvegicus (rat)

TISSUE(S): Cell Culture

SUBMITTER: Olga Bukato

LAB HEAD: Artemiy S. Silantyev

PROVIDER: PXD026776 | Pride | 2021-10-05

REPOSITORIES: Pride

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			Action	DRS
	20190627G0001_IDA_1.wiff	Wiff
	20190627G0001_IDA_1.wiff.scan	Wiff
	20190627G0001_IDA_2.wiff	Wiff
	20190627G0001_IDA_2.wiff.scan	Wiff
	20190627G0001_IDA_3.wiff	Wiff

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Profiling of glioma C6 and astrocytes rat cell lines before and after co-cultivation.

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