Proteomics

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Transporter characterisation reveals aminoethylphosphonate mineralisation as a key step in the marine phosphorus redox cycle


ABSTRACT: The planktonic synthesis of reduced organophosphorus molecules, such as alkylphosphonates and aminophosphonates, represents one half of a vast global oceanic phosphorus redox cycle. Whilst alkylphosphonates tend to accumulate in recalcitrant dissolved organic matter, aminophosphonates do not. Thus, we hypothesised unknown pathways for the uptake of aminophosphonates must exist in seawater. Here, we identify three novel bacterial 2-aminoethylphosphonate (2AEP) transporters, named AepXVW, AepP and AepSTU, whose expression is independent of phosphate concentrations (phosphate-insensitive). AepXVW is found in diverse marine heterotrophs and is ubiquitously distributed in mesopelagic and epipelagic waters. Unlike the archetypal phosphonate binding protein, PhnD, the newly identified AepX is heavily transcribed (~100-fold>PhnD) in the global ocean independently of phosphate and nitrogen concentrations, and has high specificity for 2AEP (S. stellulata AepX Kd 23±4 nM). Collectively, our data identifies a mechanism responsible for a major oxidation process in the marine phosphorus redox cycle and suggests 2AEP may be an important source of regenerated phosphate and ammonium, which are required for oceanic primary production.

INSTRUMENT(S): Orbitrap Fusion

ORGANISM(S): Stappia Stellulata Dsm 5886 Pseudomonas Putida (strain Bird-1)

SUBMITTER: Andrew Bottrill  

LAB HEAD: Dr Ian Lidbury

PROVIDER: PXD026804 | Pride | 2021-09-07

REPOSITORIES: Pride

Dataset's files

Source:
Action DRS
MS18-172.zip Other
MS18-172_Oxidation__M_Sites.txt Txt
MS18-172_allPeptides.txt Txt
MS18-172_evidence.txt Txt
MS18-172_modificationSpecificPeptides.txt Txt
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Publications

Transporter characterisation reveals aminoethylphosphonate mineralisation as a key step in the marine phosphorus redox cycle.

Murphy Andrew R J ARJ   Scanlan David J DJ   Chen Yin Y   Adams Nathan B P NBP   Cadman William A WA   Bottrill Andrew A   Bending Gary G   Hammond John P JP   Hitchcock Andrew A   Wellington Elizabeth M H EMH   Lidbury Ian D E A IDEA  

Nature communications 20210727 1


The planktonic synthesis of reduced organophosphorus molecules, such as alkylphosphonates and aminophosphonates, represents one half of a vast global oceanic phosphorus redox cycle. Whilst alkylphosphonates tend to accumulate in recalcitrant dissolved organic matter, aminophosphonates do not. Here, we identify three bacterial 2-aminoethylphosphonate (2AEP) transporters, named AepXVW, AepP and AepSTU, whose synthesis is independent of phosphate concentrations (phosphate-insensitive). AepXVW is fo  ...[more]

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