Dataset Information

LgG4 lesion of EoE patient biopsy by AutoSTOMP

ABSTRACT: To test the ability of the Automated Spatially Targeted Optical Micro Proteomics (AutoSTOMP) protocol for clinical research. We collected from a patient diagnosed with active EoE (≥15 eosinophils/hpf), according to consensus guidelines, using standard endoscopy procedures, then we try to selectively obtain the proteome from the discrete lgG4+ lesions of the biopsy sections by AutoSTOMP, and try to link the lesion structure with its specially skewed constituents comparing to the rest of the tissue.

INSTRUMENT(S): Q Exactive HF

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Esophagus

SUBMITTER: BOCHENG YIN

LAB HEAD: Sarah E. Ewald

PROVIDER: PXD026819 | Pride | 2021-08-10

REPOSITORIES: pride

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Automated Spatially Targeted Optical Microproteomics Investigates Inflammatory Lesions <i>In Situ</i>.

Yin Bocheng B Caggiano Laura R LR Li Rung-Chi RC McGowan Emily E Holmes Jeffrey W JW Ewald Sarah E SE

Journal of proteome research 20210826 9

Tissue microenvironment properties like blood flow, extracellular matrix, or proximity to immune-infiltrate are important regulators of cell biology. However, methods to study regional protein expression in the native tissue environment are limited. To address this need, we developed a novel approach to visualize, purify, and measure proteins <i>in situ</i> using automated spatially targeted optical microproteomics (AutoSTOMP). Here, we report custom codes to specify regions of heterogeneity in ...[more]

PMID: 34436902

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