Proteomics

Dataset Information

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Applying log-normal peak fitting to parallel reaction monitoring data analysis.


ABSTRACT: To support quantitative data analysis, we have developed a software, peakfit, that fits acquired chromatographic data to the log-normal peak equation and reports the calculated peak parameters. To demonstrate the capabilities of this approach, we provide hereby four example datasets: (1) 15 QCs of a PRM assay targeting the three most common isoforms of Apolipoprotein E (E2, E3, and E4); (2) PRM data of samples with 6 different ApoE phenotypes (E2/2, E2/3, E2/4, E3/3, E3/4, and E4/4); (3) shotgun run of a commercial HeLa digest to demonstrate processing of MS1 data, and (4) a quality control peptide mix to demonstrate assessment of chromatographic performance on basis of the base peak chromatogram.

INSTRUMENT(S): Orbitrap Fusion

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Cell Culture, Blood Serum

SUBMITTER: Christoph Stingl  

LAB HEAD: Theo M. Luider

PROVIDER: PXD026875 | Pride | 2021-07-26

REPOSITORIES: Pride

Dataset's files

Source:
Action DRS
210421OFc1_HeLa_031.raw Raw
210421OFc1_HeLa_031__F154475_.mzid.gz Mzid
210421OFc1_HeLa_031__F154475_.mzid_210421OFc1_HeLa_031__F154475_.MGF Mzid
210421OFc1_PM38_025.raw Raw
a166-ApoE-e22.raw Raw
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Publications

Applying Log-Normal Peak Fitting to Parallel Reaction Monitoring Data Analysis.

Stingl Christoph C   Luider Theo M TM  

Journal of proteome research 20210714 8


Chromatographic separation is often an important part of mass-spectrometry-based proteomic analysis. It reduces the complexity of the initial samples before they are introduced to mass-spectrometric detection and chromatographic characteristics (such as retention time) add analytical features to the analyte. The acquisition and analysis of chromatographic data are thus of great importance, and specialized software is used for the extraction of quantitative information in an efficient and optimiz  ...[more]

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