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Mycoplasma gallisepticum WhiA knockdown and overexpression

ABSTRACT: Proteomes of Mycoplasma gallisepticum strains with overexpression and knockdown of WhiA transcription factor. The overexpression was introduced on a transposon vector carrying M. gallisepticum whiA gene with strong constitutive promoter and strong SD sequence. The knockdown was made by CRISPRi. dCas9 protein and sgRNA against whiA gene were introduced on a transposon vector.

INSTRUMENT(S): Q Exactive Plus

ORGANISM(S): Mycoplasma Gallisepticum S6

SUBMITTER: Aleksandr Zubov

LAB HEAD: Gleb Fisunov

PROVIDER: PXD026928 | Pride | 2021-06-25

REPOSITORIES: Pride

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Dataset's files

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			Action	DRS
	LFQ_processed_Gleb.xls	Xls
	Pobeguts_dif_177_20210302_KS_OB642_dCas9_23_1_1.index	Other
	Pobeguts_dif_177_20210302_KS_OB642_dCas9_23_1_1.raw	Raw
	Pobeguts_dif_178_20210302_KS_OB643_dCas9_23_1_2.index	Other
	Pobeguts_dif_178_20210302_KS_OB643_dCas9_23_1_2.raw	Raw

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