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Sex-specific social behavior and amygdala proteomic deficits in Foxp2+/- mutant mice

ABSTRACT: In humans, mutations in the transcription factor encoding gene, FOXP2, are associated with language and Autism Spectrum (ASD) Disorders, the latter characterized by deficits in social interactions. However, little is known regarding the function of Foxp2 in male or female social behavior. Our previous studies in mice revealed high expression of Foxp2 within the medial subnucleus of the amygdala (MeA), a limbic brain region highly implicated in innate social behaviors such as mating, aggression, and parental care. Here, using a comprehensive panel of behavioral tests in male and female Foxp2+/- heterozygous mice, we investigated the role Foxp2 plays in MeA-linked innate social behaviors. We reveal significant deficits in olfactory processing, social interaction, mating, aggressive and parental behaviors. Interestingly, some of these deficits displayed in a sex-specific manner. To examine the consequences of Foxp2 loss of function specifically in the MeA, we conducted a proteomic analysis of microdissected MeA tissue and found sex differences in a host of proteins implicated in neuronal communication, connectivity and dopamine signaling. Consistent with this, we discovered that MeA Foxp2-lineage cells were responsive to dopamine with differences between males and females. Thus, our findings reveal a central and sex-specific role for Foxp2 in social behavior and MeA function.

INSTRUMENT(S): Q Exactive HF

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Brain

SUBMITTER: Maria Jesus Herrero

LAB HEAD: Joshua Corbin

PROVIDER: PXD026978 | Pride | 2021-07-07

REPOSITORIES: Pride

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			Action	DRS
	03182019-s1-4-TMT-FR1.msf	Msf
	03182019-s1-4-TMT-FR1.raw	Raw
	03182019-s1-4-TMT-FR2.raw	Raw
	03182019-s1-4-TMT-FR3.raw	Raw
	03182019-s1-4-TMT-FR4.raw	Raw

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