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Molecular insights into mechanisms of GPCR hijacking by Staphylococcus aureus

ABSTRACT: We use hydrogen-deuterium exchange mass spectrometry (HDX-MS) to analyse the effects of S. aureus leucotoxins binding to the atypical chemokine receptor ACKR1 on receptor's structure and dynamics.

INSTRUMENT(S): Synapt MS

ORGANISM(S): Homo Sapiens (human) Staphylococcus Aureus Subsp. Aureus Nn50

SUBMITTER: Cherine Bechara

LAB HEAD: Cherine Bechara

PROVIDER: PXD027043 | Pride | 2021-11-03

REPOSITORIES: Pride

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			Action	DRS
	Ion_Output.7z	Other
	WT_ACKR1_HlgAB_1.zip	Other
	WT_ACKR1_HlgAB_2.zip	Other
	deglyco_ACKR1_HlgAB_1.zip	Other

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Molecular insights into mechanisms of GPCR hijacking by <i>Staphylococcus aureus</i>.

Grison Claire M CM Lambey Paul P Jeannot Sylvain S Del Nero Elise E Fontanel Simon S Peysson Fanny F Heuninck Joyce J Sounier Rémy R Durroux Thierry T Leyrat Cédric C Granier Sébastien S Bechara Cherine C

Proceedings of the National Academy of Sciences of the United States of America 20211001 42

Atypical chemokine receptor 1 (ACKR1) is a G protein-coupled receptor (GPCR) targeted by <i>Staphylococcus aureus</i> bicomponent pore-forming leukotoxins to promote bacterial growth and immune evasion. Here, we have developed an integrative molecular pharmacology and structural biology approach in order to characterize the effect of leukotoxins HlgA and HlgB on ACKR1 structure and function. Interestingly, using cell-based assays and native mass spectrometry, we found that both components HlgA a ...[more]

PMID: 34663701

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