Dataset Information

Wheat proteome in response to Plant Growth Promoting Bacteria (PGPB) and Arbuscular Mycorrhizal Fungi (AMF) treatment

ABSTRACT: The current study is aimed at elucidating the proteomic responses in durum wheat Triticum aestivum L triggered by native PGPB, CP_4 (Bacillus subtilis) alone and in combination with AM fungi Glomus fasciculatum under field conditions Our results suggest that native PGPB B subtilis ( in combination with AM fungi Glomus fasciculatum (B+ may promote differential abundance of multiple regulatory seed storage proteins over untreated control Thus, combined application of native PGPB and AMF could offer a more sustainable approach to enhance crop yield.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Triticum Aestivum (wheat)

SUBMITTER: Radheshyam Yadav

LAB HEAD: Prof. Ramakrishna Wusirika

PROVIDER: PXD027072 | Pride | 2022-04-16

REPOSITORIES: Pride

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Wheat grain proteomic and protein-metabolite interactions analyses provide insights into plant growth promoting bacteria-arbuscular mycorrhizal fungi-wheat interactions.

Yadav Radheshyam R Chakraborty Sudip S Ramakrishna Wusirika W

Plant cell reports 20220409 6

<h4>Key message</h4>Proteomic, protein-protein and protein-metabolite interaction analyses in wheat inoculated with PGPB and AMF identified key proteins and metabolites that may have a role in enhancing yield and biofortification. Plant growth-promoting bacteria (PGPB) and arbuscular mycorrhizal fungi (AMF) have an impact on grain yield and nutrition. This dynamic yet complex interaction implies a broad reprogramming of the plant's metabolic and proteomic activities. However, little information ...[more]

PMID: 35396966

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Project description:Around two-thirds of all plant species form arbuscular mycorrhizasa symbiosis between plant roots and glomalean fungi that leads to the formation of intraradical organs of nutrient exchange and an extraradical network of fungal hyphae effectively extending the plant root system. The mycorrhiza plays a key role in plant nutrition and in enhancing plant resistance against pathogens and improving drought resistance. At present very little is known about the molecular basis of arbuscular mycorrhiza formation. Arabidopsis thaliana (as with all Brassicaceae) does not form arbuscular mycorrhizas (AM). Arabidopsis may either have lost essential gene functions or acquired new ones that prevent a successful symbiotic interaction. However given that mycorrhizal symbiosis developed very early during the evolution of land plants and that many ectomycorrhizal plant species can be colonised by AM fungi it is likely that important components of AM signalling pathways are conserved in all plants including Arabidopsis. Possibly the lack of AM development is a multigenic trait and this would make it difficult to isolate mutants that (re-)gain the ability to interact with AM fungi. What can be done however is firstly to test which parts of one or several putative AM signalling pathways are still functional and which ones are not. Secondly we can test whether negatively acting pathways such as those involved in defence against pathogenic microorganisms are induced upon inoculation with AM fungi. Together this work is likely to give important information of why Arabidopsis (and Brassicaceae) are behaving as non-hosts for AM fungi. In other words Arabidopsis will be an ideal system to study mechanisms of non-host "resistance" to AM colonisation. Elucidating these mechanisms will obviously make a great contribution to understanding the basis of the mycorrhizal interaction. Moreover using Arabidopsis as a tool it will be possible at the end to integrate the information obtained for AM signalling with that obtained for other developmental and environmentally triggered signalling pathways such as plant hormone signalling or plant defence responses. To produce an inventory of which Arabidopsis genes respond at all to inoculation with AM fungi a genome-wide screen for AM-controlled genes is proposed. RNA will be prepared from Arabidopsis roots treated with AM fungus and mock-inoculated control plants. Arabidopsis (Col-0) will be grown in pot culture (1:1 sand/Terra-Green) at low concentrations of phosphate. Three week-old plants will be inoculated with surface-sterilised spores of Gigaspora rosea. RNA will be isolated 3 days post inoculation. Experimenter name: Hsiu-Ling Yap Experimenter phone: 01904 434 302/304 Experimenter fax: 01904 434 312 Experimenter institute: University of York Experimenter address: Department of Biology University of York P.O.Box 373 York!Series_summary = Experimenter zip/postal_code: YO10 5YW Experimenter country: UK Keywords: compound_treatment_design

			Action	DRS
	AMF_rep_1.raw.raw	Raw
	AMF_rep_2.raw.raw	Raw
	CP4_AMF_rep_1.raw.raw	Raw
	CP4_AMF_rep_2.raw.raw	Raw
	CP4_rep_1.raw.raw	Raw