Dataset Information

Genome survey sequencing of Gossypium robinsonii to investigate the molecular responses of wild cotton pollen to extreme heat

ABSTRACT: Wild species are valuable resources for developing resilient crops to environmental stresses. We used Gossypium robinsonii, Australian wild cotton, to investigate the molecular signatures contributing to the tolerance of this plant to harsh environments. Three stages of pollen development, including tetrads (TE; 5-5.5mm), uninucleate microspores (UN; 7-10mm) and binucleate microspores (BN; 13-24mm) were exposed to 36/25 °C (moderate heat) or 40/30 °C (extreme heat) for 5 days, and the corresponding mature pollen grains were collected for SWATH-MS analysis. The genome of G. robinsonii assembled in the present study was used for proteome reference.

INSTRUMENT(S): TripleTOF 6600

ORGANISM(S): Gossypium Robinsonii

TISSUE(S): Plant Cell, Pollen

SUBMITTER: Farhad Masoomi-Aladizgeh

LAB HEAD: Brian J Atwell

PROVIDER: PXD027097 | Pride | 2022-02-10

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	210608_P50013_IDA_Sample_FM001.wiff	Wiff
	210608_P50013_IDA_Sample_FM001.wiff.scan	Wiff
	210608_P50013_IDA_Sample_FM002.wiff	Wiff
	210608_P50013_IDA_Sample_FM002.wiff.scan	Wiff
	210608_P50013_IDA_Sample_FM003.wiff	Wiff

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Publications

Genome survey sequencing of wild cotton (Gossypium robinsonii) reveals insights into proteomic responses of pollen to extreme heat.

Masoomi-Aladizgeh Farhad F Kamath Karthik Shantharam KS Haynes Paul A PA Atwell Brian J BJ

Plant, cell & environment 20220217 4

Heat stress specifically affects fertility by impairing pollen viability but cotton wild relatives successfully reproduce in hot savannas where they evolved. An Australian arid-zone cotton (Gossypium robinsonii) was exposed to heat events during pollen development then mature pollen was subjected to deep proteomic analysis using 57 023 predicted genes from a genomic database we assembled for the same species. Three stages of pollen development, including tetrads (TEs), uninucleate microspores (U ...[more]

PMID: 35092006

Dataset Information

Genome survey sequencing of Gossypium robinsonii to investigate the molecular responses of wild cotton pollen to extreme heat

Dataset's files

Publications

Genome survey sequencing of wild cotton (Gossypium robinsonii) reveals insights into proteomic responses of pollen to extreme heat.

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