Proteomics

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The plasma proteins from human multiple myeloma was enriched by lectin AANL6 and identified by LC-MS/MS

ABSTRACT: Lectin is widely used to enrich glycoconjugates carrying glycans and to identify the abnormal glycosylated proteins in tumor with mass spectrometry, which is a useful tool for cancer diagnosis and prognosis monitoring. In our previously reported, we identified a novel lectin AANL from Agrocybe aegerita that has a higher affinity than other lectins when binding with GlcNAc, and the lectin AANL has been used for early diagnosis of non-small cell lung cancer. Subsequently, AANL6, a mutant of AANL, is cloned and purified, and has higher affinity to terminal GlcNAc than AANL. Therefore, in this study, we used the lectin AANL6 for enrichment of glycosylated peoteins from MM, and identified with quantitative proteomics, in order to find a potential sensitive biomarker for MM diagnosis.

INSTRUMENT(S): TripleTOF 5600

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Blood Plasma

SUBMITTER: Yanxia Jin  

LAB HEAD: Yanxia Jin

PROVIDER: PXD027111 | Pride | 2024-05-22

REPOSITORIES: Pride

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Action DRS
AAL26_enriched_peptides_in_MM_PeptideSummary.txt Txt
AAL26_enriched_proteins_in_MM_ProteinSummary.txt Txt
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Publications

Identification of novel combined biomarkers in the diagnosis of multiple myeloma.

Jin Yanxia Y   Liang Yuxing Y   Su Yanting Y   Hui Lingyun L   Liu Hailing H   Ding Lu L   Zhou Fuling F  

Hematology (Amsterdam, Netherlands) 20211201 1

<h4>Purpose</h4>Multiple myeloma (MM) is a haematological malignant disease with a clonal proliferation of plasma cells, and timely surveillance is helpful to improve the survival rate of patients with MM. However, there is a lack of simple and effective biomarkers for the diagnosis, prognosis, and residual disease evaluation of MM.<h4>Material & methods</h4>In the detection cohort, we used the samples from six newly diagnosed MM patients and six control subjects. Plasma proteins were labelled w  ...[more]

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