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Novel recognition site for polyUb and UBL signals in Rpn1

ABSTRACT: Rpn1 is the largest proteasomal receptor for polyubiquitin and shuttle proteins contains one known binding site for polyubiquitin and shuttle proteins. We used a combination of NMR spectroscopy, photo-crosslinking, mass spectrometry, and mutagenesis,to confirm the presence of additional ubiquitin recognition site on RPN1. This novel site is situated in the N-terminal section of Rpn1, a region previously surmised to be devoid of functionality.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Homo Sapiens (human) Saccharomyces Cerevisiae (baker's Yeast)

SUBMITTER: Oded Kleifeld

LAB HEAD: David Fushman

PROVIDER: PXD027128 | Pride | 2021-08-09

REPOSITORIES: Pride

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Dataset's files

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			Action	DRS
	DiUb_K11-BpA-RPN1Short_Feb2021Spectra_all.pdf	Pdf
	DiUb_K48-BpA-RPN1Short_Feb2021Spectra_all.pdf	Pdf
	DiUb_K63-BpA-RPN1Short_Feb2021Spectra_all.pdf	Pdf
	Escherichia_coli_Uni_Fumsan_pLink_con.fasta	Fasta
	Fusman_PlinkQ49ecoli_con.fasta	Fasta

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