Dataset Information

Sequence specific recognition of U1 snRNP stem-loop 4 by the ubiquitin-like domain of U2 snRNP protein SF3A1 and its RGG motif

ABSTRACT: Cross-linking of isotope-labelled RNA coupled with mass spectrometry (CLIR-MS) was used to study the interaction between the ubiquitin-like domain (UBL) of the protein SF3A1, a component of the U2 snRNP, with stem-loop 4 RNA (SL4) from the U1 snRNP. The complex was cross-linked and analysed both in isolation, and in broader context with components of the U1 snRNP. Cross-linking was performed using irradiation under 254 nm light, relying on the inherent reactivity of ribonucleotides.

INSTRUMENT(S): Orbitrap Fusion Lumos, LTQ Orbitrap Elite

ORGANISM(S): Homo Sapiens (human)

SUBMITTER: Christopher Sarnowski

LAB HEAD: Alexander Leitner

PROVIDER: PXD027189 | Pride | 2022-02-22

REPOSITORIES: Pride

ACCESS DATA

Dataset's files

Source:

			Action	DRS
	201017_LU02_csarnowski_2016_NC10A1.raw	Raw
	201017_LU02_csarnowski_2016_NC10A2.raw	Raw
	201201_LU02_csarnowski_2018_NC10Ca.raw	Raw
	201201_LU02_csarnowski_2018_NC10Cb.raw	Raw
	201201_LU02_csarnowski_2020_SF3U1a.raw	Raw

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Publications

Sequence-specific RNA recognition by an RGG motif connects U1 and U2 snRNP for spliceosome assembly.

de Vries Tebbe T Martelly William W Campagne Sébastien S Sabath Kevin K Sarnowski Chris P CP Wong Jason J Leitner Alexander A Jonas Stefanie S Sharma Shalini S Allain Frédéric H-T FH

Proceedings of the National Academy of Sciences of the United States of America 20220201 6

In mammals, the structural basis for the interaction between U1 and U2 small nuclear ribonucleoproteins (snRNPs) during the early steps of splicing is still elusive. The binding of the ubiquitin-like (UBL) domain of SF3A1 to the stem-loop 4 of U1 snRNP (U1-SL4) contributes to this interaction. Here, we determined the 3D structure of the complex between the UBL of SF3A1 and U1-SL4 RNA. Our crystallography, NMR spectroscopy, and cross-linking mass spectrometry data show that SF3A1-UBL recognizes, ...[more]

PMID: 35101980

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