Dataset Information

Arabidopsis hy2 mutant LC-MS/MS

ABSTRACT: Salt stress is a critical factor of abiotic stress in agricultural production that significantly affects seed germination and early seedling development. In this study, we characterized the function of the Arabidopsis Long Hypocotyl 2(HY2) gene in NaCl signaling. The hy2 mutant was NaCl-insensitive, whereas HY2-overexpressing lines shows NaCl-hypersensitive phenotypes during seed germination. Induced by exogenous NaCl, the transcription and the protein level of HY2 were up regulated, and HY2 positively mediated the expression of downstream stress-related genes of RD29A, RD29B and DREB2A. With further quantitative proteomics, we got the patterns of 7,391 proteins under salt stress, and identified 215 differentially regulated proteins (DRPs) specifically regulated by HY2. According to GO enrichment analysis, these proteins are mainly involved in ion homeostasis, hormone response, reactive oxygen species(ROS) metabolic, photosynthesis and detoxification pathway to response salt stress . These results direct the pathway of HY2 participating salt stress, and provide new insights for the plant to resist salt stress.

INSTRUMENT(S): Orbitrap Fusion Lumos

ORGANISM(S): Arabidopsis Thaliana (mouse-ear Cress)

TISSUE(S): Whole Body

SUBMITTER: Jinpeng Zou

LAB HEAD: Nan Yao

PROVIDER: PXD027204 | Pride | 2022-02-17

REPOSITORIES: Pride

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The Arabidopsis HY2 Gene Acts as a Positive Regulator of NaCl Signaling during Seed Germination.

Piao Mingxin M Zou Jinpeng J Li Zhifang Z Zhang Junchuan J Yang Liang L Yao Nan N Li Yuhong Y Li Yaxing Y Tang Haohao H Zhang Li L Yang Deguang D Yang Zhenming Z Du Xinglin X Zuo Zecheng Z

International journal of molecular sciences 20210820 16

Phytochromobilin (PΦB) participates in the regulation of plant growth and development as an important synthetase of photoreceptor phytochromes (phy). In addition, Arabidopsis long hypocotyl 2 (HY2) appropriately works as a key PΦB synthetase. However, whether HY2 takes part in the plant stress response signal network remains unknown. Here, we described the function of HY2 in NaCl signaling. The hy2 mutant was NaCl-insensitive, whereas HY2-overexpressing lines showed NaCl-hyp ...[more]

PMID: 34445714

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Project description:Arabidopsis ecotypes of Sha and Ler showed differences in tolerance to salinity stress. A previous study indicated that a premature stop codon resulting in a truncated Response to ABA and Salt 1 (RAS1) protein in Sha contributes to the increased salt tolerance relative to Ler ecotype. Sha exhibited higher germination rates and longer roots on MS plate, presumably due to the decreased ABA sensitivity in Sha. More Sha plants also survived in soil after salt treatment with relatively lower electrolyte leakage when compared to Ler. Transcriptome analysis revealed that expression levels of many genes were changed between Sha and Ler ecotypes and by salt treatments. About 500 transcripts were commonly changed by at least one salinity effect and one ecotype effect, and 171 of them were co-regulated by all four comparisons. Transcripts involved in redox, secondary metabolism, auxin metabolism, photosynthesis, cell wall, and protein synthesis were mainly down-regulated by salinity effects, while transposable element genes, microRNA and antisense sequences, histone superfamily genes, and biotic stress related genes were significantly changed by Sha ecotype effects and only slightly by salinity. Several metabolic pathways such as stress, TCA, hormone/lipid/secondary metabolism, redox, development, and GO terms involved in stress, oxidation, and defense response were enriched by both salinity and ecotype effects. Ninety-five highly inducible genes were identified as candidates of RAS1 target genes and the functions involved hormone metabolism, biotic stress, RNA, DNA synthesis, protein metabolism, cell, and microRNA metabolism. All these results indicated that the Sha ecotype was possibly preconditioned to abiotic stress relative to Ler through regulation of signaling pathways and stress responsive gene expression. These comparative transcriptomic and analytical results also confirm the complexity of ABA responses and salt stress tolerance mechanisms, and they suggest additional targets for improving tolerance. Ten days old seedlings of two Arabidopsis ecotypes, Sha and Ler, were treated with 100 mM NaCl on MS plate. Plant materials were collected for RNA extraction at 4th days after treatments.

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Dataset Information

Arabidopsis hy2 mutant LC-MS/MS

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Publications

The <i>Arabidopsis HY2</i> Gene Acts as a Positive Regulator of NaCl Signaling during Seed Germination.

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