Proteomics

Dataset Information

0

Identification of novel salivary candidate protein biomarkers for tuberculosis diagnosis: a preliminary biomarker discovery study.


ABSTRACT: Abstract Background There is an urgent need for new, accurate, rapid, and affordable tuberculosis (TB) diagnostic tests. The aim of the present study was to use mass spectrometry to identify new preliminary candidate TB diagnostic protein biomarkers in saliva obtained from individuals with TB, and patients with other respiratory diseases (ORD). Methods Saliva samples were collected from 22 individuals who self-presented with symptoms suggestive of TB as part of a larger TB biomarker project. Purified salivary proteins were subjected to tryptic digestion peptides were analysed using a QExactive Orbitrap Mass Spectrometer. Identified proteins were subjected to gene ontology and ingenuity pathway analysis for functional enrichment analysis. Results 26 of the 652 identified proteins significantly discriminated individuals with TB from those with ORD after Benjamini Hochberg correction (5% FDR), with five of these proteins diagnosing TB with an AUC ≥ 0.80. A 5-protein biosignature comprising of P01011, Q8NCW5, P28072, A0A2Q2TTZ9, and Q99574 diagnosed TB with an AUC of 1.00 (95% CI, 1.00-1.00), sensitivity of 100% (95% CI, 76.2-100%) and specificity of 90.9% (95% CI, 58.7-99.8%) after leave-one-out cross validation. Conclusions We identified novel candidate salivary protein biomarkers and biosignatures with strong potential as TB diagnostic candidates. Our results are preliminary and require validation in larger studies.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Saliva

SUBMITTER: Hygon Mutavhatsindi  

LAB HEAD: Professor Jonathan Blackburn

PROVIDER: PXD027294 | Pride | 2021-08-05

REPOSITORIES: Pride

Dataset's files

Source:
Action DRS
190426_HM_A1.raw Raw
190426_HM_A_340.raw Raw
190426_HM_B.raw Raw
190426_HM_B_360.raw Raw
190426_HM_C.raw Raw
Items per page:
1 - 5 of 41

Similar Datasets

2016-03-10 | PXD003251 | Pride
2018-11-06 | PXD004653 | Pride
2013-04-01 | E-MEXP-3618 | biostudies-arrayexpress
2022-02-16 | PXD024216 | Pride
2024-02-06 | GSE255074 | GEO
2024-02-06 | GSE255073 | GEO
2024-02-06 | GSE255071 | GEO
2022-10-15 | PXD033830 | Pride
2019-06-30 | GSE69579 | GEO
2018-12-05 | GSE98550 | GEO