Proteomics

Dataset Information

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Venomics of the desert black cobra Walterinnesia morgani from Çörten village, Turkey


ABSTRACT: Combined transcriptomics-assisted bottom-up and top-down MS characterization of the venom proteome of the desert black cobra Walterinnesia morgani from Çörten village, Turkey

INSTRUMENT(S): Synapt MS

ORGANISM(S): Serpentes

SUBMITTER: Davinia Pla  

LAB HEAD: Juan J Calvete

PROVIDER: PXD027497 | Pride | 2022-02-17

REPOSITORIES: Pride

Dataset's files

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Publications

Combined Molecular and Elemental Mass Spectrometry Approaches for Absolute Quantification of Proteomes: Application to the Venomics Characterization of the Two Species of Desert Black Cobras, <i>Walterinnesia aegyptia</i> and <i>Walterinnesia morgani</i>.

Calvete Juan J JJ   Pla Davinia D   Els Johannes J   Carranza Salvador S   Damm Maik M   Hempel Benjamin-Florian BF   John Elisa B O EBO   Petras Daniel D   Heiss Paul P   Nalbantsoy Ayse A   Göçmen Bayram B   Süssmuth Roderich D RD   Calderón-Celis Francisco F   Nosti Alicia Jiménez AJ   Encinar Jorge Ruiz JR  

Journal of proteome research 20211004 11


We report a novel hybrid, molecular and elemental mass spectrometry (MS) setup for the absolute quantification of snake venom proteomes shown here for two desert black cobra species within the genus <i>Walterinnesia</i>, <i>Walterinnesia aegyptia</i> and <i>Walterinnesia morgani</i>. The experimental design includes the decomplexation of the venom samples by reverse-phase chromatography independently coupled to four mass spectrometry systems: the combined bottom-up and top-down molecular MS for  ...[more]

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