Proteomics

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Adsorptive microtiter plates as solid supports in affinity purification workflows

ABSTRACT: Affinity ligands such as antibodies are widely used in (bio)medical research for purifying proteins from complex biological samples. These ligands are generally immobilized onto solid supports which facilitate the separation of captured protein from sample matrix. Adsorptive microtiter plates are commonly used as solid supports prior to immunochemical detection (e.g. immunoassays) but hardly ever prior to LC-MS-based detection, and here we describe some applications, opportunities, and challenges of corresponding workflows.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Murine Leukemia Virus Isolates

TISSUE(S): Macrophage Cell Line

SUBMITTER: Horvatovich Péter  

LAB HEAD: Peter Horvatovich

PROVIDER: PXD027530 | Pride | 2021-11-25

REPOSITORIES: Pride

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FKBP5-mAB-RC1.mzID.gz Mzid
FKBP5-mAB-RC1.mzML Mzml
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FKBP5-mAB-RC2.mzID.gz Mzid
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Adsorptive Microtiter Plates As Solid Supports in Affinity Purification Workflows.

Klont Frank F   Kwiatkowski Marcel M   Faiz Alen A   van den Bosch Thea T   Pouwels Simon D SD   Dekker Frank J FJ   Ten Hacken Nick H T NHT   Horvatovich Péter P   Bischoff Rainer R  

Journal of proteome research 20211020 11

Affinity ligands such as antibodies are widely used in (bio)medical research for purifying proteins from complex biological samples. These ligands are generally immobilized onto solid supports which facilitate the separation of a captured protein from the sample matrix. Adsorptive microtiter plates are commonly used as solid supports prior to immunochemical detection (e.g., immunoassays) but hardly ever prior to liquid chromatography-mass spectrometry (LC-MS-)-based detection. Here, we describe  ...[more]

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