Alteration in liver proteome caused by ethinyl estradiol in males Cyprinodon variegatus
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ABSTRACT: Adult male marine fish were exposed to 100ng/L of ethinyl estradiol (EE2) for 7 days in 1.8L vessels, dimethyl sulfoxide (DMSO) was used as carrier never reaching concentrations > 0.001% v/v. The same volume of DMSO was added to the solvent control vessels. Culture conditions, monitored daily, were as follows: temperature 18.8 ± 0.2°C, pH 7.94±0.03, dissolved oxygen saturation 99.3% ± 1.1 and 16:8 hours of light:dark photoperiod. 90% of the water was renewed daily and EE2 and DMSO concentrations were adjusted after that.
INSTRUMENT(S): LTQ Orbitrap Elite
ORGANISM(S): Cyprinodon Variegatus
TISSUE(S): Liver
SUBMITTER: Alexandre M Schönemann
LAB HEAD: Angel P. Diz
PROVIDER: PXD027547 | Pride | 2022-05-23
REPOSITORIES: Pride
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