Proteomics

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Integrated metabolomics and proteomics dynamics of serum samples reveals dietary Zeolite clinoptilolite supplementation restores energy balance in high yielding dairy cows


ABSTRACT: Dairy cows can suffer from a negative energy balance (NEB) during their transition from dry period to early lactation when feed intake does not sufficiently meet the energy requirements for body maintenance and homeostasis. The subsequent metabolic changes can increase the risk of postpartum diseases such as clinical ketosis, mastitis and fatty liver. Zeolite clinoptilolite (CPL), due to its ion-exchange property, has been often used to treat NEB and other disorders such as rumen digestion in animals in farming. However, limited information is available on the dynamics of global metabolomics and proteomic profiles in serum that could give us a better understanding on the associated altered biological pathways in response to CPL. Thus, in the present study, a total 64 cows randomly assigned to two groups: control (n=32) and CPL-treated (n=32) at time points of 30 days (n=8) and 10 days (n=8) prepartum stage and 5 days (n=8) and 26 (n=8) days postpartum stage. Labelled proteomics and untargeted metabolomics were performed following the determination of β-hydroxy-butyric acid (BHB) concentration in the samples indicating NEB. A total of 64 proteins were differentially expressed with a significant cohort appearing to play key roles in restoring the EB after CPL supplementation. In addition, 21 differentially expressed metabolites were chosen of 83 identified metabolites based on their high correlation with BHB. Joint pathway and interaction analysis revealed cross-talks among potential candidates such as valproic acid, leucic acid, glycerol, fibronectin and kinninogen-1 which could be responsible for restoring associated complications of NEB such as infertility or infection like mastitis. By using global proteomics and metabolomics strategy, the present study concluded that the CPL supplementation could lower NEB and restore energy balance in just a few weeks and explained the possible underlying pathways employed by the CPL.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Bos Taurus (bovine)

TISSUE(S): Blood Serum

SUBMITTER: Sudipa Maity  

LAB HEAD: Vladimir Mrljak

PROVIDER: PXD027551 | Pride | 2022-02-17

REPOSITORIES: pride

Dataset's files

Source:
Action DRS
TMTsix10_cow.raw Raw
TMTsix11_cow.raw Raw
TMTsix12_cow.raw Raw
TMTsix13_cow.raw Raw
TMTsix1_cow.msf Msf
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Integrated Metabolomics and Proteomics Dynamics of Serum Samples Reveals Dietary Zeolite Clinoptilolite Supplementation Restores Energy Balance in High Yielding Dairy Cows.

Maity Sudipa S   Rubić Ivana I   Kuleš Josipa J   Horvatić Anita A   Đuričić Dražen D   Samardžija Marko M   Ljubić Blanka Beer BB   Turk Romana R   Gračner Damjan D   Maćešić Nino N   Valpotić Hrvoje H   Mrljak Vladimir V  

Metabolites 20211205 12


Dairy cows can suffer from a negative energy balance (NEB) during their transition from the dry period to early lactation, which can increase the risk of postpartum diseases such as clinical ketosis, mastitis, and fatty liver. Zeolite clinoptilolite (CPL), due to its ion-exchange property, has often been used to treat NEB in animals. However, limited information is available on the dynamics of global metabolomics and proteomic profiles in serum that could provide a better understanding of the as  ...[more]

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