Proteomics

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LC-MS/MS data of Extracellular Vimentin

ABSTRACT: Angiotensin-converting enzyme 2 (ACE2) has been identified as a key entry receptor for SARS-CoV-2. The involvement of other cellular components that partake in viral entry appears to be likely but is not yet fully understood. Here we describe the identification of vimentin (VIM), an intermediate filament protein widely expressed in cells of mesenchymal origin, as an important attachment factor for SARS-CoV-2 on human endothelial cells. Using liquid chromatography–tandem mass spectrometry (LC-MS/MS), we identified VIM as a protein that binds to the SARS-CoV-2 spike (S) protein.

INSTRUMENT(S): Orbitrap Fusion Lumos

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Endothelial Cell

SUBMITTER: Chaoshuang Xia  

LAB HEAD: Catherine E. Costello

PROVIDER: PXD027595 | Pride | 2022-02-22

REPOSITORIES: Pride

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Dataset's files

Source:
Action DRS
103020_Control_Binding_fraction_90min_method.raw Raw
103020_Control_washing_fraction_90min_method.raw Raw
103020_IP_Binding_fraction_90min_method_20201030174033.raw Raw
103120_IP_washing_fraction_90min_method.raw Raw
20201215_ControlSample_B1.raw Raw
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Publications

Extracellular vimentin is an attachment factor that facilitates SARS-CoV-2 entry into human endothelial cells.

Amraei Razie R   Xia Chaoshuang C   Olejnik Judith J   White Mitchell R MR   Napoleon Marc A MA   Lotfollahzadeh Saran S   Hauser Blake M BM   Schmidt Aaron G AG   Chitalia Vipul V   Mühlberger Elke E   Costello Catherine E CE   Rahimi Nader N  

Proceedings of the National Academy of Sciences of the United States of America 20220201 6

SARS-CoV-2 entry into host cells is a crucial step for virus tropism, transmission, and pathogenesis. Angiotensin-converting enzyme 2 (ACE2) has been identified as the primary entry receptor for SARS-CoV-2; however, the possible involvement of other cellular components in the viral entry has not yet been fully elucidated. Here we describe the identification of vimentin (VIM), an intermediate filament protein widely expressed in cells of mesenchymal origin, as an important attachment factor for S  ...[more]

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