Proteomics

Dataset Information

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Interferon Regulatory Factor 6 (IRF6) knockout in human keratinocytes disrupts epithelial homeostasis


ABSTRACT: The transcription factor Interferon Regulatory Factor 6 (IRF6) is crucially involved in craniofacial development and regulates the proliferation/differentiation balance in keratinocytes. Pathological IRF6 variants have been found in Van der Woude syndrome (VWS), the most common syndromic form of cleft lip / palate (CLP) as well as in non-syndromic CLP cases. Besides its prominent function in regulating keratinocyte differentiation, recent data revealed that IRF6 is also involved in wound healing and migration. Since a significant fraction of CLP patients undergoing corrective cleft surgery experience wound healing complications, IRF6 represents an interesting candidate gene linking the two processes. However, Irf6 function has been mainly studied in mice and knowledge on IRF6 in human cells remains sparse. Here, we aimed to elucidate the role of IRF6 in human postnatal skin- and oral mucosa-derived keratinocytes by its ablation using CRISPR/Cas9. We complement this approach by applying proteomics and identify that lack of IRF6 disrupts human epithelial homeostasis by altering cell colony morphology, migration pattern, and the differentiation potential of keratinocytes.

INSTRUMENT(S): Orbitrap Fusion Lumos

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Primary Cell, Keratinocyte, Skin

SUBMITTER: Manfred Heller  

LAB HEAD: Martin Degen

PROVIDER: PXD027669 | Pride | 2022-02-17

REPOSITORIES: Pride

Dataset's files

Source:
Action DRS
20200930_KO_O_IRFG_cl15_2_EG_i01.raw Raw
20200930_KO_O_IRFG_cl15_2_EG_i02.raw Raw
20200930_KO_O_IRFG_cl26_1_EG_i01.raw Raw
20200930_KO_O_IRFG_cl26_1_EG_i02.raw Raw
20200930_WT_OKFG_3_EG_i01.raw Raw
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Publications

Lack of IRF6 Disrupts Human Epithelial Homeostasis by Altering Colony Morphology, Migration Pattern, and Differentiation Potential of Keratinocytes.

Girousi Eleftheria E   Muerner Lukas L   Parisi Ludovica L   Rihs Silvia S   von Gunten Stephan S   Katsaros Christos C   Degen Martin M  

Frontiers in cell and developmental biology 20210930


Variants within the gene encoding for the transcription factor Interferon Regulatory Factor 6 (IRF6) are associated with syndromic and non-syndromic Cleft Lip/Palate (CLP) cases. IRF6 plays a vital role in the regulation of the proliferation/differentiation balance in keratinocytes and is involved in wound healing and migration. Since a fraction of CLP patients undergoing corrective cleft surgery experience wound healing complications, <i>IRF6</i> represents an interesting candidate gene linking  ...[more]

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