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Global and mitochondrial proteomics under GSH depletion

ABSTRACT: Glutathione (GSH) is a tripeptide thiol abundantly present in all eukaryotes1. As the major site of oxidative metabolism, mitochondria must maintain sufficient levels of GSH in order to perform key protective and biosynthetic functions. GSH is exclusively synthesized in the cytosol, yet how it enters mitochondria and the molecular machinery involved in mitochondrial GSH import remain elusive. Here, using mass-spectrometry approaches, we identify SLC25A39, a mitochondrial membrane carrier of unknown function, to mediate GSH transport into mitochondria. SLC25A39 loss reduces mitochondrial GSH import and abundance, without impacting whole cell GSH levels.

INSTRUMENT(S): Orbitrap Fusion Lumos

ORGANISM(S): Homo Sapiens (human)

SUBMITTER: Soren Heissel

LAB HEAD: Kivanc Birsoy

PROVIDER: PXD027673 | Pride | 2021-11-15

REPOSITORIES: Pride

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			Action	DRS
	MS206363LUM_Birsoy_Target.raw	Raw
	MS206363LUM_Ross_Birsoy_SPS-MS3_TMT_fraction02.raw	Raw
	MS206363LUM_Ross_Birsoy_SPS-MS3_TMT_fraction03.raw	Raw
	MS206363LUM_Ross_Birsoy_SPS-MS3_TMT_fraction04.raw	Raw
	MS206363LUM_Ross_Birsoy_SPS-MS3_TMT_fraction05.raw	Raw

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