Dataset Information

Expansion of the Drosophila melanogaster sperm proteome

ABSTRACT: Given the continued advances in mass spectrometry technology and methods for database searching since the previous characterization of the Drosophila melanogaster sperm proteome, a new proteomic analysis of sperm samples was conducted to expand the size and coverage of the sperm proteome. This dataset is part of a larger project examining the molecular life history of Drosophila sperm.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Drosophila Melanogaster (fruit Fly)

TISSUE(S): Spermatozoon

SUBMITTER: Erin McCullough

LAB HEAD: Steve Dorus

PROVIDER: PXD027808 | Pride | 2022-03-14

REPOSITORIES: Pride

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Publications

The life history of <i>Drosophila</i> sperm involves molecular continuity between male and female reproductive tracts.

McCullough Erin L EL Whittington Emma E Singh Akanksha A Pitnick Scott S Wolfner Mariana F MF Dorus Steve S

Proceedings of the National Academy of Sciences of the United States of America 20220307 11

SignificanceIn species with internal fertilization, sperm spend an important part of their lives within the female. To examine the life history of the sperm during this time, we used semiquantitative proteomics and sex-specific isotopic labeling in fruit flies to determine the extent of molecular continuity between male and female reproductive tracts and provide a global catalog of sperm-associated proteins. Multiple seminal fluid proteins and female proteins associate with sperm immediately aft ...[more]

PMID: 35254899

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			Action	DRS
	0314wa7s_6_1A_1.mgf	Mgf
	0314wa7s_6_1A_1.raw	Raw
	0314wb7s_6_1A_2.mgf	Mgf
	0314wb7s_6_1A_2.raw	Raw
	0314wc7s_6_1A_3.mgf	Mgf