Label-free LC-MS/MS of Ubiquitinated proteins derived from UPs-4T1/WT and UPs-4T1/EPB nanovaccine
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ABSTRACT: In this project, 4T1 parental cells (4T1/WT) were exposed to increasing concentrations of epirubicin (EPB) to establish a novel multi-drug resistant CSC-like breast cancer cell line (4T1/EPB). The ubiquitinated proteins were enriched from 4T1/WT or 4T1/EPB derived cell lysate using a-Al2O3-Vx3 nanoparticles to produce the covalently linked product UPs nanovaccine. Label-free LC-MS/MS mass spectrometry was used to detect the type and amount of enriched proteins of UPs from the 4T1/WT cells and the 4T1/EPB cells.
INSTRUMENT(S): TripleTOF 5600
ORGANISM(S): Mus Musculus (mouse)
TISSUE(S): Stem Cell, Cell Culture
DISEASE(S): Breast Cancer
SUBMITTER: Fang Huang
LAB HEAD: Fang Huang
PROVIDER: PXD027822 | Pride | 2021-08-20
REPOSITORIES: Pride
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