Dataset Information

HHbFPS1 may play an important role in the effect of tapping panel dryness on the natural rubber biosynthesis in Hevea brasiliensis latex

ABSTRACT: Tapping panel dryness (TPD) seriously affects the natural rubber (NR) production of Hevea brasiliensis (rubber tree). Several studies have speculated that TPD influences NR biosynthesis in the latex of rubber trees based on the expression changes of NR biosynthesis-related genes. In this study, iTRAQ analysis of latex were carried out to reveal the molecular mechanism of TPD affecting rubber trees NR biosynthesis activity and molecular weight.

INSTRUMENT(S): TripleTOF 5600

ORGANISM(S): Hevea Brasiliensis

TISSUE(S): Latex

SUBMITTER: Zhiyi Nie

LAB HEAD: Zhiyi Nie

PROVIDER: PXD027892 | Pride | 2023-05-10

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	1_all.mgf	Mgf
	2_all.mgf	Mgf
	3_all.mgf	Mgf
	GDI1645-Hevea_brasiliensis_rubber-9-iTRAQ_sub_1.csv	Csv
	GDI1645-Hevea_brasiliensis_rubber-9-iTRAQ_sub_1.overall.xls	Xls

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Publications

Downregulation of HbFPS1 affects rubber biosynthesis of Hevea brasiliensis suffering from tapping panel dryness.

Nie Zhiyi Z Kang Guijuan G Yan Dong D Qin Huaide H Yang Lifu L Zeng Rizhong R

The Plant journal : for cell and molecular biology 20230109 3

Tapping panel dryness (TPD) is a century-old problem that has plagued the natural rubber production of Hevea brasiliensis. TPD may result from self-protective mechanisms of H. brasiliensis in response to stresses such as excessive hormone stimulation and mechanical wounding (bark tapping). It has been hypothesized that TPD impairs rubber biosynthesis; however, the underlying mechanisms remain poorly understood. In the present study, we firstly verified that TPD-affected rubber trees exhibited lo ...[more]

PMID: 36524729

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Project description:Purpose: de novo sequencing and comparative analysis of the bark transciptomes of Hevea brasiliensis induced without ethephon (C), with ethephon for 8 hours (E8) and 24 hours (E24) to identify the genes and pathways related to the stimulation of rubber production by ethylene. The goals of this study are to reveal the molecular mechanism behind the stimulation of rubber production by ethylene. Methods: Bark RNA was extracted using the TRIzolÂ® Reagent (Invitrogen) and two cDNA libraries, H (healthy rubber trees) and T (TPD-affected trees), were prepared using the mRNA-Seq 8 sample prep Kit (Illumina). The libraries were deep sequenced using Illumina HiSeqTM 2000 (Illumina Inc., San Diego, CA, USA). Raw reads produced from sequencing machines were resorted to de novo assembly and gene annotation. Results: De novo sequencing and assembly of the bark transciptomes of Hevea brasiliensis induced with ethephon for 8 hours (E8) and 24 hours (E24) were performed. 51,965,770, 52,303,714 and 53,177,976 high-quality clean reads from E8, E24 and C (control) samples were assembled into 81,335, 80,048 and 80,800 unigenes respectively, with a total of 84,425 unigenes and an average length of 1,101 bp generated. 10,216 and 9,374 differentially expressed genes (DEGs) in E8 and E24 compared with C were respectively detected. The expression of several enzymes in crucial points of regulation in glycolysis were up-regulated and DEGs were not significantly enriched in isopentenyl diphosphate (IPP) biosynthesis pathway. In addition, up-regulated genes of great regulatory importance in carbon fixation (Calvin cycle) were identified. Conclusions: The rapid acceleration of glycolytic pathway supplying precursors for the biosynthesis of IPP and natural rubber, instead of rubber biosynthesis per se, may be responsible for ethylene stimulation of latex yield in rubber tree. The elevated rate of flux throughout the Calvin cycle may account for some durability of ethylene-induced stimulation. Our finding lays the foundations for molecular diagnostic and genetic engineering for high-yielding improvement of rubber tree. De novo sequencing of the transcriptomes of C (bark without ethephon application), E8 (bark with 1.5%-ethephon treatment for 8 hours) and E24 (bark with 1.5%-ethephon treatment for 24 hours) rubber trees was conducted using Illumina HiSeq 2000.