Dataset Information

Yorkie and Bonus protein interactome-Hippo pathway and Bonus control developmental cell fate decisions in the Drosophila eye

ABSTRACT: To identify novel regulators of the Hippo pathway, we performed affinity purification-mass spectrometry (AP-MS) using Drosophila embryos overexpressing Yki-EGFP with a ubiquitous driver da-GAL4, as well as cultured S2 cells expressing Yki-SBP. We identified the core Hippo pathway components, multiple Hippo pathway regulators and functional groups, and several putative Yki interactors including Bonus (Bon). To identify additional cofactors that are recruited by Bon, we performed AP-MS using Bon-SBP expressed in Drosophila S2 cells. Further genetic tests revealed the involvement of Bon interactors, HDAC1, Su(var)2-10, and Hrb27C, in the Drosophila eye specification that is regulated by the Yki-Bon complex.

INSTRUMENT(S): LTQ Orbitrap

ORGANISM(S): Drosophila Melanogaster (fruit Fly)

TISSUE(S): Embryo, Cell Culture

SUBMITTER: Heya Zhao

LAB HEAD: Alexey Veraksa

PROVIDER: PXD027934 | Pride | 2023-01-17

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	10_Yki-SBP_1st <75kDa_peptide.csv	Csv
	10_Yki-SBP_1st <75kDa_protein.csv	Csv
	11_Yki-SBP_2nd >75kDa_peptide.csv	Csv
	11_Yki-SBP_2nd >75kDa_protein.csv	Csv
	12_Yki-SBP_2nd <75kDa_peptide.csv	Csv

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Publications

Hippo pathway and Bonus control developmental cell fate decisions in the Drosophila eye.

Zhao Heya H Moberg Kenneth H KH Veraksa Alexey A

Developmental cell 20230302 5

The canonical function of the Hippo signaling pathway is the regulation of organ growth. How this pathway controls cell-fate determination is less well understood. Here, we identify a function of the Hippo pathway in cell-fate decisions in the developing Drosophila eye, exerted through the interaction of Yorkie (Yki) with the transcriptional regulator Bonus (Bon), an ortholog of mammalian transcriptional intermediary factor 1/tripartite motif (TIF1/TRIM) family proteins. Instead of controlling t ...[more]

PMID: 36868234

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