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Proteomic analysis in S100A16-overexpressed human gastric cancer cells lines

ABSTRACT: Our studies reveal that S100A16 overexpression triggers GC cells proliferation and migration both in vivo and in vitro; by contrast, S100A16 knockdown restricts the speed of GC cells growth and mobility. Proteomic analysis results reveal a large S100A16 interactome, which includes ZO-2 (Zonula Occludens-2), a master regulator of cell-to-cell tight junction

INSTRUMENT(S): TripleTOF 5600

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Epithelial Cell, Cell Culture

DISEASE(S): Stomach Cancer

SUBMITTER: Xiaoying You

LAB HEAD: Dongming Su

PROVIDER: PXD028022 | Pride | 2022-02-17

REPOSITORIES: Pride

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			Action	DRS
	IPS100-SGC7901-11-20180429.raw	Raw
	IPS100-SGC7901-OE-12-20180429.raw	Raw
	IgG-SGC7901-5-20180429.raw	Raw
	IgG-SGC7901-OE-6-20180429.raw	Raw
	S100A16-identification.xlsx	Xlsx

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Publications

Calcium Binding Protein S100A16 Expedites Proliferation, Invasion and Epithelial-Mesenchymal Transition Process in Gastric Cancer.

You Xiaoying X Li Min M Cai Hongwei H Zhang Wenwen W Hong Ye Y Gao Wenjie W Liu Yun Y Liang Xiubin X Wu Tijun T Chen Fang F Su Dongming D

Frontiers in cell and developmental biology 20210928

Gastric cancer (GC) is one of the most common malignant tumors of the digestive system, listed as the second cause of cancer-related deaths worldwide. S100 Calcium Binding Protein A16 (S100A16) is an acidic calcium-binding protein associated with several types of tumor progression. However, the function of S100A16 in GC is still not very clear. In this study, we analyzed S100A16 expression with the GEPIA database and the UALCAN cancer database. Meanwhile, 100 clinical GC samples were used for th ...[more]

PMID: 34650982

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Quantitative proteomic Analysis Reveals up-regulation of caveolin-1 in FOXP3-overexpressed human gastric cancer cells

Project description:Forkhead box protein 3 (FOXP3) is implicated in tumor progression and prognosis in various types of tumor cells. We have recently reported that FOXP3 inhibited proliferation of gastric cancer (GC) cells through activating the apoptotic signaling pathway. In this study, we found that over-expression of FOXP3 inhibited GC cell migration, invasion and proliferation. Then, the label-free quantitative proteomic approach was employed to further investigating the down-stream proteins regulated by FOXP3, resulting in a total of 3,978 proteins quantified, including 186 significantly changed proteins. Caveolin-1 (CAV1), as a main constituent protein of caveolae, was one of those changed proteins up-regulated in FOXP3-overexpressed GC cells, moreover, it was assigned as one of the node proteins in the protein-protein interaction network and the key protein involved in focal adhesion pathway by bioinformatics analysis. Further biological experiments confirmed that FOXP3 directly bound to the promoter regions of CAV1 to positively regulate CAV1 transcription in GC cells. In summary, our study suggested that FOXP3 can be considered as a tumor suppressor in GC via positively regulating CAV1 through transcriptional activation, and this FOXP3-CAV1 transcriptional regulation axis may play an important role in inhibiting invasion and metastasis of GC cells.

2017-11-03 | PXD007725 | Pride

Examination of HMGA2 binding genes in human HMGA2-exogenous overexpressed gastric cancer cells

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Expression analysis of gastric cancer cells treated with periplocin

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Quantitative proteomic Analysis Reveals up-regulation of caveolin-1 inFOXP3-overexpressed human gastric cancer cells

Project description:Forkhead box protein 3 (FOXP3) is associated with tumor progression and prognosis in various types of tumor cells. We have recently reported that FOXP3 inhibited proliferation of gastric cancer (GC) cells through activating the apoptotic signaling pathway. In this study, we found that over-expression of FOXP3 inhibited GC cell migration, invasion and proliferation. To further investigate the down-stream proteins regulated by FOXP3, the label-free quantitative proteomic approach was employed to analyze the gastric cells stably transfected with FOXP3-containing lentivirus or empty vector. A total of 3,978 proteins were identified and quantified, including 186 significantly changed proteins. Caveolin-1 (CAV1) was one of those changed proteins up-regulated in FOXP3-overexpressed gastric cells, moreover, it is one of the node proteins in the protein-protein interaction network and the key protein involved in focal adhesion pathway by bioinformatics analysis. As the main constituent of caveolae, caveolin-1 interacts directly with several signaling molecules and acts as a tumor suppressor in numerous carcinomas. Further biological experiments confirmed that FOXP3 directly binded to the promoter regions of CAV1 to positively regulated CAV1 transcription in GC cells. In summary, our study suggested that FOXP3 plays a tumor-suppressing role in GC via positively regulating CAV1 through transcriptional activation, and this FOXP3-CAV1 transcriptional regulation ais may play an important role in inhibiting invasion and metastasis of GC cells.

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