Proteomics

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Identification and function of components from a divergent mRNA export pathway in trypanosomes

ABSTRACT: mRNA export is central to gene regulation and expression. In trypanosomes, transcription is polycistronic, all mRNAs are processed by trans-splicing and export is mediated by non-canonical mechanisms. We have described several conserved mRNA export pathway components in Trypanosoma cruzi, including orthologs of Sub2, a component of the TREX complex, and eIF4AIII, a core component of the exon junction complex (EJC). Few orthologs of proteins involved in mRNA export in higher eukaryotes are detectable in trypanosome genomes and examples of mechanistic divergence well known. To uncover additional components of the trypanosome mRNA maturation and export system we undertook an unbiased search for protein interactors of TcSub2 and TceIF4AIII. Significant overlap between TcSub2 and TceIF4AIII interacting protein cohorts suggests that both proteins associate with similar machinery. We confirmed several interactions with conserved core components of the EJC and multiple additional complexes, together with identification of proteins specific to trypanosomatids. The highly interactive super-interactome uncovered here, capable of supporting RNA processing from splicing through to nuclear export and cytoplasmic events, highlights kinetoplastid-specific and conserved components creating an amalgam to support the unique mRNA maturation mechanisms in trypanosomes.

INSTRUMENT(S): LTQ Orbitrap XL ETD

ORGANISM(S): Trypanosoma Cruzi Dm28c

SUBMITTER: Patricia Domingues  

LAB HEAD: Andréa Rodrigues Ávila

PROVIDER: PXD028072 | Pride | 2022-02-07

REPOSITORIES: Pride

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Dataset's files

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Action DRS
20150626_Haruo_Mex67_1.RAW Raw
20150626_Haruo_WT_1.RAW Raw
20150729_Haruo_WT_2.RAW Raw
20150729_Mex67_2.RAW Raw
20160307_Haruo_Mex67_3.RAW Raw
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Publications

Proteomics Uncovers Novel Components of an Interactive Protein Network Supporting RNA Export in Trypanosomes.

Inoue Alexandre Haruo AH   Domingues Patricia Ferreira PF   Serpeloni Mariana M   Hiraiwa Priscila Mazzocchi PM   Vidal Newton Medeiros NM   Butterfield Erin R ER   Del Pino Ricardo Canavate RC   Ludwig Adriana A   Boehm Cordula C   Field Mark C MC   Ávila Andréa Rodrigues AR  

Molecular & cellular proteomics : MCP 20220126 3

In trypanosomatids, transcription is polycistronic and all mRNAs are processed by trans-splicing, with export mediated by noncanonical mechanisms. Although mRNA export is central to gene regulation and expression, few orthologs of proteins involved in mRNA export in higher eukaryotes are detectable in trypanosome genomes, necessitating direct identification of protein components. We previously described conserved mRNA export pathway components in Trypanosoma cruzi, including orthologs of Sub2, a  ...[more]

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