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Ubiquitination of DEAD-box RNA helicase DDX3X by TRIM25

ABSTRACT: Tripartite motif protein 25 (TRIM25) is an E3 ligase that ubiquitinates multiple substrates within the RLR signalling cascade and plays both RING (really interesting new gene)-dependent and RING-independent roles in RIG-I-mediated IFN induction. We report that the PRY-SPRY domain of TRIM25 interacts with the N-terminal extension (NTE) of DEAD-box helicase 3X (DDX3X), a host protein with multiple roles in RLR signalling. Gene reporter assays and knockdown studies reveal DDX3X and TRIM25 cooperate to activate the IFN- promoter following RIG-I activation independent of DDX3X’s catalytic activity. We also show that TRIM25 ubiquitinates DDX3X at several lysine residues in vitro and in cells.

INSTRUMENT(S): LTQ Orbitrap XL

ORGANISM(S): Homo Sapiens (human)

SUBMITTER: Oded Kleifeld

LAB HEAD: Natalie Borg

PROVIDER: PXD028122 | Pride | 2021-09-08

REPOSITORIES: Pride

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			Action	DRS
	2020-10-29-decoys-reviewed-contam-UP000005640.fas	Other
	Seq30746_E1O1.RAW	Raw
	Seq30746_E1O1.mzML	Mzml
	Seq30746_E1O1.pepXML	Pepxml
	fragger.params	Other

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Publications

TRIM25 and DEAD-Box RNA Helicase DDX3X Cooperate to Regulate RIG-I-Mediated Antiviral Immunity.

Atkinson Sarah C SC Heaton Steven M SM Audsley Michelle D MD Kleifeld Oded O Borg Natalie A NA

International journal of molecular sciences 20210823 16

The cytoplasmic retinoic acid-inducible gene-I (RIG-I)-like receptors (RLRs) initiate interferon (IFN) production and antiviral gene expression in response to RNA virus infection. Consequently, RLR signalling is tightly regulated by both host and viral factors. Tripartite motif protein 25 (TRIM25) is an E3 ligase that ubiquitinates multiple substrates within the RLR signalling cascade, playing both ubiquitination-dependent and -independent roles in RIG-I-mediated IFN induction. However, addition ...[more]

PMID: 34445801

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circNDUFB2 inhibits non-small cell lung cancer progression via destabilizing IGF2BPs and activating anti-tumor immunity

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TRIM25

Project description:TRIM25

| PRJNA316328 | ENA

The long noncoding RNA Lnczc3h7a promotes TRIM25-mediated RIG-I antiviral innate immune response

Project description:The long noncoding RNA Lnczc3h7a promotes TRIM25-mediated RIG-I antiviral innate immune response

| PRJNA443151 | ENA

RNA-binding activity of TRIM25 is mediated by its PRY/SPRY domain and is required for ubiquitination

Project description:TRIM25 E3 ubiquitin ligase is a new RNA-binding protein. We present the first high-throughput analysis of the molecular interactomes of TRIM25. We show that TRIM25 is a bona fide RNA-binding protein that interacts with numerous coding and non-coding transcripts. This suggests that TRIM25 could play a role in the regulation of RNA metabolism.

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DDX3X regulation of global translation is impaired by medulloblastoma-associated mutations [RiboSeq]

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