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Exopalaemon carinicauda in response to alkalinity stress

ABSTRACT: In this study, an integrative analysis of the gill-specific proteome at 0 h, 12 h and 36 h after alkalinity stress was performed to identify important regulators and pathways involved in alkalinity adaption of Exopalaemon carinicauda. This study reveals the first time-course, gill-specific, combined proteomic profiling associated with alkalinity adaption of E. carinicauda and provides new insights into the mechanisms underlying the molecular response to alkalinity stress in shrimp.

INSTRUMENT(S): Q Exactive HF

ORGANISM(S): Palaemon Carinicauda

TISSUE(S): Gill

SUBMITTER: Qianqian Ge

LAB HEAD: Jitao Li

PROVIDER: PXD028137 | Pride | 2021-11-02

REPOSITORIES: Pride

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Project description:The aim of this experiment was to explore transcriptomic changes in the gills of Altantic salmon (Salmo salar) following a model lab based parr smolt transformation from fresh water (FW) to salt water (SW). The process of smoltification (migration from FW to SW) is stimulated by long day photoperiod, which acts on the tissue-specific levels of active thyroid hormone (triiodothyronine, T3) through the expression of thyroid hormone type 2 deiodinase (dio2), responsible for conversion of inactive thyroxine (T4) to T3. To gain insight into the functional significance of dio2 induction, we performed a SW-challenge experiment in which we inhibited dio2 activity by addition of iopanoic acid (IOP) to the SW. We also assessed the ability of co-treatment with T3 to override IOP effects. Juvenile fish maintained in FW were subjected to a standard smolt photoperiod regime known to stimulate smoltification, after which they were randomly allocated to one of the four treatments: SW, SW+IOP, SW+IOP+T3 and FW as a control. Fish (n = 8-10 per treatment) were exposed to these conditions for 6 h and then sacrificed to obtain gill tissue for microarray analysis, carried out using a custom-designed Agilent oligonucleotide microarray platform Salar_2 (one glass slide with 4 x 44K arrays, Agilent design ID: 025520, array design A-MEXP-2065), developed and validated for Atlantic salmon (for details, see specific protocols) . In total, 36 hybridisations were performed on gills from individual fish, with 8-10 replicate fish per treatment. We identified 1939 genes whose expression was significantly increased or decreased by transfer from FW to SW. For a subset of 259 genes, this SW response was abolished if IOP was added to the SW, but maintained if T3 was also present during IOP treatment. This group of genes constitutes a candidate list, for which SW-inducibility appears to be dependent on locally mediated changes in gill T3 availability. The results of this experiment have been submitted for publication in Current Biology and are currently under review.

			Action	DRS
	Dj21LMD_bjhb1_.pep.xml	Pepxml
	Dj21LMD_bjhb1_.prot.xml	Xml
	Dj21LMD_bjhb1_1.raw	Raw
	Dj21LMD_bjhb1_10.raw	Raw
	Dj21LMD_bjhb1_11.raw	Raw

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Exopalaemon carinicauda in response to alkalinity stress

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