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De novo formation of apical-basal polarity by Rap1 regulates epiblast patterning

ABSTRACT: The epiblast is the first cell type that forms apical-basal polarity de novo as the mouse embryo implants into the maternal uterus, while the extraembryonic neighbours of the epiblast - trophectoderm and primitive endoderm - retain their pre-established polarity beyond implantation [1]; however, it is still unclear how the epiblast establishes apical-basal polarity de novo. Here, we focused on Rap1 signaling pathway, which is activated during the transition of the epiblast from the naïve to primed state of pluripotency during implantation [2]. Through the preestablished in vitro three-dimensional culture system [3], genetic knockouts and proximity-biotinylation analyses, we found that Rap1 integrates multiple signals that contribute to de novo formation of apical-basal polarity. Importantly, formation of apical-basal polarity in the epiblast is essential for its correct patterning and proper communication with the extraembryonic lineages. Altogether, these results not only dissect molecular details of de novo apical-basal polarity formation, but also have broader implications for epithelial polarity and development.

INSTRUMENT(S): Q Exactive HF

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Embryo, Embryonic Stem Cell

SUBMITTER: Hannes Drexler

LAB HEAD: Hannes C. A. Drexler

PROVIDER: PXD028315 | Pride | 2022-06-14

REPOSITORIES: Pride

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	20200911_exp1063_Justin_BioID_07.raw	Raw
	20200911_exp1063_Justin_BioID_08.raw	Raw
	20200911_exp1063_Justin_BioID_09.raw	Raw
	20200911_exp1063_Justin_BioID_10.raw	Raw
	20200911_exp1063_Justin_BioID_11.raw	Raw

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