Proteomics

Dataset Information

0

Transcriptome and proteome analysis in LUHMES cells overexpressing alpha-synculein

ABSTRACT: LUHMES cells share many characteritics with human dopamingeric neurons in the substantia nigra, the cells whose demise is responsible for the motor symptoms in Parkinson’s disease (PD). LUHMES cells can therefore be used bona fide as a model to study pathophysiological processes involved in PD. Previously, we showed that LUHMES cell degenerate after six days upon overexpression of wild type alpha-synuclein. In the present study we performed a transcriptome and proteome expression analysis in alpha-synuclein-overexpressing cells and GFP-expressing control cells in order to identify genes and proteins that are differentially regulated upon overexpression of alpha-synuclein. The analysis was performed four days after the initiation of alpha-synuclein or GFP overexpression, before the cells died in order to identify processes that preceded cell death.

INSTRUMENT(S): Q Exactive HF

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Brain

DISEASE(S): Parkinson's Disease

SUBMITTER: Markus Stepath  

LAB HEAD: PD Dr. Martin Eisenacher

PROVIDER: PXD028322 | Pride | 2022-05-19

REPOSITORIES: Pride

Json Xml

Dataset's files

Source:
Action DRS
QEXHF08343.mzML Mzml
QEXHF08343.mzid.gz Mzid
QEXHF08343.raw Raw
QEXHF08345.mzML Mzml
QEXHF08345.mzid.gz Mzid
Items per page:
1 - 5 of 56
altmetric image

Publications

Transcriptome and Proteome Analysis in LUHMES Cells Overexpressing Alpha-Synuclein.

Höllerhage Matthias M   Stepath Markus M   Kohl Michael M   Pfeiffer Kathy K   Chua Oscar Wing Ho OWH   Duan Linghan L   Hopfner Franziska F   Eisenacher Martin M   Marcus Katrin K   Höglinger Günter U GU  

Frontiers in neurology 20220411

LUHMES cells share many characteristics with human dopaminergic neurons in the substantia nigra, the cells, the demise of which is responsible for the motor symptoms in Parkinson's disease (PD). LUHMES cells can, therefore, be used <i>bona fide</i> as a model to study pathophysiological processes involved in PD. Previously, we showed that LUHMES cells degenerate after 6 days upon overexpression of wild-type alpha-synuclein. In the present study, we performed a transcriptome and proteome expressi  ...[more]

Similar Datasets

Proteomics Reproducibility, specificity and accuracy of DIA quantification - DDA analysis
2019-11-08 | PXD012986 | Pride
Transcriptomics Phenotypic manifestation of alpha-synuclein strains from Parkinson’s disease and multiple system atrophy in human dopaminergic neruons
2020-09-02 | GSE149632 | GEO
Transcriptomics Expression data from oligodendroglial cell line
2013-10-28 | E-GEOD-51781 | biostudies-arrayexpress
Proteomics Low-Density Lipoprotein Receptor fuels the cellular cholesterol-mTORC1 axis required for CD8+ T cell activation
2022-09-20 | PXD030365 | Pride
Proteomics Mouse liver proteome fractionation using leptin-based cartridges.
2021-07-30 | PXD025097 | Pride
Genomics α-Synuclein promotes H4R3me2s through interacting with BAF-PRMT5 complex
2023-11-23 | GSE228331 | GEO
Other RNA Bind-N-Seq for purified α-Synuclein and BG4 (scFV for G-quadruplex)
2023-07-17 | GSE235418 | GEO
Transcriptomics Expression data from oligodendroglial cell line
2013-10-28 | GSE51781 | GEO
Transcriptomics Expression data from Parkison's iPSCs with four copies of SNCA, and equivalent cell lines from an unaffected first degree relative
2011-09-14 | E-GEOD-30792 | biostudies-arrayexpress
Transcriptomics Expression data from fibroblasts, iPSCs and neurons with four copies of SNCA, and equivalent cell lines from an unaffected first degree relative
2011-09-14 | E-GEOD-28365 | biostudies-arrayexpress