Proteomics

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Transcriptome and proteome analysis in LUHMES cells overexpressing alpha-synculein

ABSTRACT: LUHMES cells share many characteritics with human dopamingeric neurons in the substantia nigra, the cells whose demise is responsible for the motor symptoms in Parkinson’s disease (PD). LUHMES cells can therefore be used bona fide as a model to study pathophysiological processes involved in PD. Previously, we showed that LUHMES cell degenerate after six days upon overexpression of wild type alpha-synuclein. In the present study we performed a transcriptome and proteome expression analysis in alpha-synuclein-overexpressing cells and GFP-expressing control cells in order to identify genes and proteins that are differentially regulated upon overexpression of alpha-synuclein. The analysis was performed four days after the initiation of alpha-synuclein or GFP overexpression, before the cells died in order to identify processes that preceded cell death.

INSTRUMENT(S): Q Exactive HF

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Brain

DISEASE(S): Parkinson's Disease

SUBMITTER: Markus Stepath  

LAB HEAD: PD Dr. Martin Eisenacher

PROVIDER: PXD028322 | Pride | 2022-05-19

REPOSITORIES: Pride

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Publications

Transcriptome and Proteome Analysis in LUHMES Cells Overexpressing Alpha-Synuclein.

Höllerhage Matthias M   Stepath Markus M   Kohl Michael M   Pfeiffer Kathy K   Chua Oscar Wing Ho OWH   Duan Linghan L   Hopfner Franziska F   Eisenacher Martin M   Marcus Katrin K   Höglinger Günter U GU  

Frontiers in neurology 20220411

LUHMES cells share many characteristics with human dopaminergic neurons in the substantia nigra, the cells, the demise of which is responsible for the motor symptoms in Parkinson's disease (PD). LUHMES cells can, therefore, be used <i>bona fide</i> as a model to study pathophysiological processes involved in PD. Previously, we showed that LUHMES cells degenerate after 6 days upon overexpression of wild-type alpha-synuclein. In the present study, we performed a transcriptome and proteome expressi  ...[more]

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