Dataset Information

Human sperm tails proteomics - Centrosome enrichment

ABSTRACT: The aim of this study was to identify the human sperm tail proteome using normozoospermic samples. In particular, we were interested in identifying the centrosomal proteins present in the human sperm.

INSTRUMENT(S): LTQ Orbitrap Velos

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Sperm

SUBMITTER: Farners Amargant i Riera

LAB HEAD: Isabelle Vernos

PROVIDER: PXD028348 | Pride | 2022-02-15

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	170327_S_FAIV_01_01_45pto.msf	Msf
	170327_S_FAIV_01_01_45pto.raw	Raw
	170327_S_FAIV_02_01_45pto.msf	Msf
	170327_S_FAIV_02_01_45pto.raw	Raw
	170327_S_FAIV_03_01_45pto.msf	Msf

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Publications

The human sperm basal body is a complex centrosome important for embryo preimplantation development.

Amargant Farners F Pujol Aïda A Ferrer-Vaquer Anna A Durban Mercè M Martínez Meritxell M Vassena Rita R Vernos Isabelle I

Molecular human reproduction 20211101 11

The mechanism of conversion of the human sperm basal body to a centrosome after fertilization, and its role in supporting human early embryogenesis, has not been directly addressed so far. Using proteomics and immunofluorescence studies, we show here that the human zygote inherits a basal body enriched with centrosomal proteins from the sperm, establishing the first functional centrosome of the new organism. Injection of human sperm tails containing the basal body into human oocytes followed by ...[more]

PMID: 34581808

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