Dataset Information

Hypoxic Incubation Conditions for Optimized Manufacture of Tenocyte-Based Active Substances of Homologous Standardized Transplant Products in Tendon Regenerative Medicine

ABSTRACT: Human fetal progenitor tenocytes (hFPT) produced in defined cell bank systems have recently been characterized and qualified as potential therapeutic cell sources in tendon regenerative medicine. In view of further optimizing the manufacture of the cell-based active substance, the effects of hypoxic (i.e., 2% O2 partial pressure) in vitro culture expansion on key cellular characteristics or process parameters were evaluated. To this end, multiple aspects were comparatively assessed, in either normoxic incubation (i.e., 5% CO2 and 21% O2, standard conditions) or in hypoxic incubation (i.e., 5% CO2 and 2% O2, optimized conditions). Experimentally investigated parameters and endpoints comprised cellular proliferation, cellular morphology and size distribution, cell surface marker panels, cell susceptibility toward adipogenic and osteogenic induction, while relative protein expression levels were analyzed by quantitative mass spectrometry. Results outlined conserved basic cellular characteristics (i.e., surface marker panels, phenotype under chemical induction) as well as modified key cellular parameters (i.e., cell size distribution, endpoint cell yields, matrix protein contents) potentially procuring tangible benefits with regard to an optimized cell manufacturing workflow. Specific proteomic analyses further shed some light on the effects of hypoxia at a protein level, potentially orienting the further processing of hFPT for cell-free active substance manufacture. Overall, this study indicated that hypoxic incubation impacts specific hFPT key properties and enables optimized manufacture of tenocyte-based active substances in homologous standardized transplant products.

INSTRUMENT(S): Orbitrap Exploris 480

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Tenocyte

SUBMITTER: Romain Hamelin

LAB HEAD: Alexis Laurent

PROVIDER: PXD028359 | Pride | 2022-02-15

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	AJ_200917_Expl_TMT_OFFGel_01.raw	Raw
	AJ_200917_Expl_TMT_OFFGel_02.raw	Raw
	AJ_200917_Expl_TMT_OFFGel_03.raw	Raw
	AJ_200917_Expl_TMT_OFFGel_04.raw	Raw
	AJ_200917_Expl_TMT_OFFGel_05.raw	Raw

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Publications

Hypoxic Incubation Conditions for Optimized Manufacture of Tenocyte-Based Active Pharmaceutical Ingredients of Homologous Standardized Transplant Products in Tendon Regenerative Medicine.

Jeannerat Annick A Peneveyre Cédric C Armand Florence F Chiappe Diego D Hamelin Romain R Scaletta Corinne C Hirt-Burri Nathalie N de Buys Roessingh Anthony A Raffoul Wassim W Applegate Lee Ann LA Laurent Alexis A

Cells 20211025 11

Human fetal progenitor tenocytes (hFPT) produced in defined cell bank systems have recently been characterized and qualified as potential therapeutic cell sources in tendon regenerative medicine. In view of further developing the manufacture processes of such cell-based active pharmaceutical ingredients (API), the effects of hypoxic in vitro culture expansion on key cellular characteristics or process parameters were evaluated. To this end, multiple aspects were comparatively assessed in normoxi ...[more]

PMID: 34831095

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