Dataset Information

Single muscle fiber proteomics reveals remodeling of focal adhesions in response to muscle unloading and microgravity

ABSTRACT: Spaceflight causes loss of muscle mass and strength, metabolic remodeling and insulin resistance, contributing to severe challenges for astronaut health. We used highly sensitive proteomics to detail single fiber type-specific molecular remodeling caused by muscle unloading in subjects undergoing prolonged bed rest. We then measured the muscle proteome of astronauts before and after a mission on the ISS. Our combined datasets show downregulation of complexes involved in fiber-matrix interaction, force transmission and insulin receptor stabilization on the sarcolemma. Unloading upregulated anti-oxidant responses in slow but not in fast fibers, markers of neuromuscular damage and the hypusination pathway that uniquely modifies translation initiation factor 5A. These muscle intrinsic changes in adhesion, protein turnover and redox balance may contribute to the whole-body detrimental effects caused by prolonged unloading. We conclude that single muscle fiber analysis by proteomics can support the development of advanced countermeasures targeting the mechano-metabolic molecular axis.

INSTRUMENT(S): timsTOF Pro

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Skeletal Muscle

SUBMITTER: Mario Oroshi

LAB HEAD: Marta Murgia

PROVIDER: PXD028435 | Pride | 2023-03-10

REPOSITORIES: Pride

ACCESS DATA

Dataset's files

Source:

			Action	DRS
	MARSPRE_SDRF_sample_to_data_file_format.txt	Txt
	MARSPRE_sample_to_data_file_format.csv	Csv
	MARSPRE_sample_to_data_file_format.xlsx	Xlsx
	METADATA_annotation.txt	Txt
	Raw_Astronauts.7z	Other

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Publications

Signatures of muscle disuse in spaceflight and bed rest revealed by single muscle fiber proteomics.

Murgia Marta M Ciciliot Stefano S Nagaraj Nagarjuna N Reggiani Carlo C Schiaffino Stefano S Franchi Martino V MV Pišot Rado R Šimunič Boštjan B Toniolo Luana L Blaauw Bert B Sandri Marco M Biolo Gianni G Flück Martin M Narici Marco V MV Mann Matthias M

PNAS nexus 20220611 3

Astronauts experience dramatic loss of muscle mass, decreased strength, and insulin resistance, despite performing daily intense physical exercise that would lead to muscle growth on Earth. Partially mimicking spaceflight, prolonged bed rest causes muscle atrophy, loss of force, and glucose intolerance. To unravel the underlying mechanisms, we employed highly sensitive single fiber proteomics to detail the molecular remodeling caused by unloading and inactivity during bed rest and changes of the ...[more]

PMID: 36741463

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