Proteomics

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A SISCAPA-based approach for detection of SARS-CoV-2 viral antigens from clinical samples.

ABSTRACT: SARS-CoV-2 has created a global disease burden infecting >100 million humans in just over a year. In this study, we adopted a SISCAPA-based enrichment approach using anti-peptide antibodies generated against peptides from the nucleocapsid protein of SARS-CoV-2. We developed a targeted workflow in which nasopharyngeal samples were digested followed by enrichment of viral peptides using the anti-peptide antibodies and targeted parallel reaction monitoring (PRM) analysis using a high-resolution mass spectrometer. This workflow was applied to 41 RT-PCR-confirmed clinical nasopharyngeal swab samples and 30 negative samples. The SISCAPA-based platform described in the current study can serve as one of the alternative methods for SARS-CoV-2 viral detection.

INSTRUMENT(S): Orbitrap Eclipse

ORGANISM(S): Homo Sapiens (human)

DISEASE(S): Covid-19

SUBMITTER: Akhilesh Pandey  

LAB HEAD: Akhilesh Pandey

PROVIDER: PXD028505 | Pride | 2022-02-16

REPOSITORIES: Pride

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02-11-2021_SISCAPA_LOD_100fmol_01.raw Raw
02-11-2021_SISCAPA_LOD_100fmol_02.raw Raw
02-11-2021_SISCAPA_LOD_100fmol_03.raw Raw
02-11-2021_SISCAPA_LOD_125amol_01.raw Raw
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SARS-CoV-2, a novel human coronavirus, has created a global disease burden infecting > 100 million humans in just over a year. RT-PCR is currently the predominant method of diagnosing this viral infection although a variety of tests to detect viral antigens have also been developed. In this study, we adopted a SISCAPA-based enrichment approach using anti-peptide antibodies generated against peptides from the nucleocapsid protein of SARS-CoV-2. We developed a targeted workflow in which nasopharyn  ...[more]

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