Proteomics

Dataset Information

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Liquid chromatograph-mass spectrometry based on the iTRAQ approach identified the differentially expressed proteins of two different drought-resistant maize varieties under moderate drought stress conditions and explained the physiological response mechanisms


ABSTRACT: Drought stress, especially during the seedling stage, seriously limits the growth and development of maize. Understanding the response of maize to drought is the first step in the breeding of tolerant genotypes. Recent advances in deep-sequencing and proteomic techniques, such as isobaric tags for relative and absolute quantitation (iTRAQ), can provide large-scale comparisons and reliable quantitative measurements. Despite previous studies on drought resistance mechanisms by which maize cope with water deficient, the link between physiological and molecular variations are largely unknown. Therefore, understanding the drought tolerance mechanisms of different maize varieties is essential for genetic manipulation and/or cross breeding in maize. Towards this goal, we used a comparative physiological and proteomics analysis approach to monitor the changes of two different drought-resistant maize varieties.

INSTRUMENT(S): Q Exactive HF

ORGANISM(S): Zea Mays (maize)

TISSUE(S): Leaf

SUBMITTER: qiu siqi  

LAB HEAD: Renhe Zhang

PROVIDER: PXD028508 | Pride | 2022-02-16

REPOSITORIES: Pride

Dataset's files

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Action DRS
F001381.dat Other
F001382.dat Other
F001394.dat Other
Yumiye_R1_1.raw Raw
Yumiye_R1_10.raw Raw
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Publications

Physiological and proteomic analyses revealed the response mechanisms of two different drought-resistant maize varieties.

Li Hongjie H   Yang Mei M   Zhao Chengfeng C   Wang Yifan Y   Zhang Renhe R  

BMC plant biology 20211104 1


<h4>Background</h4>Drought stress severely limits maize seedling growth and crop yield. Previous studies have elucidated the mechanisms by which maize acquires drought resistance and contends with water deficiency. However, the link between the physiological and molecular variations among maize cultivars are unknown. Here, physiological and proteomic analyses were conducted to compare the stress responses of two maize cultivars with contrasting drought stress tolerance.<h4>Results</h4>The physio  ...[more]

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