Dataset Information

Identification of plasma extracellular vesicle biomarkers after burn injury

ABSTRACT: Severe burn injury is a one of the most devastating forms of trauma with over 1.1 million burns each year requiring medical attention in the United States. Deaths from burn injury are commonly caused by immune-related sequelae such as pneumonia, organ failure and other opportunistic bacterial infections. Though there have been numerous studies to assess the immunological dysfunction associated with burn injury, there have yet to be a predictive biomarker, that can be used to assess high risk patients and their outcomes. We hypothesized that circulating extracellular vesicles (EVs) released early after burn injury would promote activate peripheral macrophages and specific cargo could be used as a biomarker to identify at-risk patients. To test this hypothesis, we assessed the immune consequences of adoptive transfer of EVs isolated after burn injury in vitro and used unbiased proteomic on EVs from mouse models and human burn patients from the UNC Jaycee Burn Center. Findings here suggest EVs serve as mediators of immune dysfunction and potential biomarkers.

INSTRUMENT(S): Q Exactive HF

ORGANISM(S): Homo Sapiens (human) Mus Musculus (mouse)

TISSUE(S): Blood Plasma

SUBMITTER: Laura Herring

LAB HEAD: Leon Coleman

PROVIDER: PXD028515 | Pride | 2022-02-16

REPOSITORIES: Pride

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Dataset's files

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			Action	DRS
	20190513_PC590-Maile_S1.raw	Raw
	20190513_PC590-Maile_S10.raw	Raw
	20190513_PC590-Maile_S11.raw	Raw
	20190513_PC590-Maile_S12.raw	Raw
	20190513_PC590-Maile_S2.raw	Raw

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Publications

Burn Injury Induces Proinflammatory Plasma Extracellular Vesicles That Associate with Length of Hospital Stay in Women: CRP and SAA1 as Potential Prognostic Indicators.

Maile Robert R Willis Micah L ML Herring Laura E LE Prevatte Alex A Mahung Cressida C Cairns Bruce B Wallet Shannon S Coleman Leon G LG

International journal of molecular sciences 20210918 18

Severe burn injury is a devastating form of trauma that results in persistent immune dysfunction with associated morbidity and mortality. The underlying drivers of this immune dysfunction remain elusive, and there are no prognostic markers to identify at-risk patients. Extracellular vesicles (EVs) are emerging as drivers of immune dysfunction as well as biomarkers. We investigated if EVs after burn injury promote macrophage activation and assessed if EV contents can predict length of hospital st ...[more]

PMID: 34576246

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