Proteomics

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Comparative proteomic analysis of plasma membrane proteins in rice leaves reveals a vesicle trafficking network in plant immunity provoked by blast fungi

ABSTRACT: This project aims to analyze rice plasma membrane proteins related to resistance against rice blast infection. We extracted rice plasma membrane proteins before and after M.oryzae infection for 24h, and then used trypsin to digest and iTRAQ to label the peptides, HPLC-MS/MS was used to seperate and identify peptides. 1.1/0.909 fold change with p-value < 0.05 was used as threshold for differentially expressed proteins. 2,977 proteins were identified and 951 of which were found to be responsive to resistance against M. oryzae. Gene Ontology (GO) enrichment analysis, Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis and protein interaction network showed that plenty of proteins were involved in vesicle trafficking with obvious functional tendencies towards transport, vesicle-mediated transport, secretion, endocytosis and phagosome. 10 DEPs were validated at transcript level, and a SNARE protein named NPSN (novel plant-specific SNARE)13 actively responded to M. oryzae infection, and it contributed to rice blast resistance and mainly located at PM.

INSTRUMENT(S): Q Exactive HF

ORGANISM(S): Oryza Sativa (rice)

TISSUE(S): Leaf

SUBMITTER: Zhi Zhao  

LAB HEAD: Yongmei Bao

PROVIDER: PXD028545 | Pride | 2022-05-19

REPOSITORIES: Pride

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Comparative Proteomic Analysis of Plasma Membrane Proteins in Rice Leaves Reveals a Vesicle Trafficking Network in Plant Immunity That Is Provoked by Blast Fungi.

Zhao Zhi Z   Li Meng M   Zhang He H   Yu Yao Y   Ma Lu L   Wang Wei W   Fan Yunxin Y   Huang Ning N   Wang Xinying X   Liu Kunquan K   Dong Shinan S   Tang Haijuan H   Wang Jianfei J   Zhang Hongsheng H   Bao Yongmei Y  

Frontiers in plant science 20220425

Rice blast, caused by <i>Magnaporthe oryzae</i>, is one of the most devastating diseases in rice and can affect rice production worldwide. Rice plasma membrane (PM) proteins are crucial for rapidly and precisely establishing a defense response in plant immunity when rice and blast fungi interact. However, the plant-immunity-associated vesicle trafficking network mediated by PM proteins is poorly understood. In this study, to explore changes in PM proteins during <i>M. oryzae</i> infection, the P  ...[more]

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