Dataset Information

Proteomic analysis of different pgr5 mutants and pgrl1ab in Arabidopsis thaliana

ABSTRACT: PGR5 is a small chloroplast protein that performs cyclic electron flow (CEF) around photosystem I and it is required for the proton gradient generation across the thylakoid membrane. The level of this protein is closely regulated by PGRL1. However, the molecular mechanism of PGR5 is still elusive. In this work, we have generated a new pgr5 mutant in Arabidopsis using CRISPR-Cas9 technology, pgr5-Cas, completely devoid of PGR5. We performed comparative proteomic analysis on pgr5-1 and pgr5-Cas lines (#1 and #2), as well as on pgrl1ab mutant.

INSTRUMENT(S): impact II

ORGANISM(S): Arabidopsis Thaliana (mouse-ear Cress)

SUBMITTER: Giada Marino

LAB HEAD: Dario Leister

PROVIDER: PXD028563 | Pride | 2022-08-12

REPOSITORIES: Pride

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Publications

Commonalities and specialties in photosynthetic functions of PROTON GRADIENT REGULATION5 variants in Arabidopsis.

Penzler Jan-Ferdinand JF Marino Giada G Reiter Bennet B Kleine Tatjana T Naranjo Belen B Leister Dario D

Plant physiology 20221001 3

The PROTON GRADIENT REGULATION5 (PGR5) protein is required for trans-thylakoid proton gradient formation and acclimation to fluctuating light (FL). PGR5 functionally interacts with two other thylakoid proteins, PGR5-like 1 (PGRL1) and 2 (PGRL2); however, the molecular details of these interactions are largely unknown. In the Arabidopsis (Arabidopsis thaliana) pgr5-1 mutant, the PGR5G130S protein accumulates in only small amounts. In this work, we generated a knockout allele of PGR5 (pgr5-Cas) us ...[more]

PMID: 35946785

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			Action	DRS
	MaxQuant_Output_Database_Param.7z	Other
	Sample_list.xlsx	Xlsx
	pgr5-1_vs_Col5.7z	Other
	pgrl1_pgr5-cas_vs_Col0.7z	Other