Proteomics

Dataset Information

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Tyrosine Phosphorylation of NLRP3 by Src Family Protein Tyrosine Kinase Lyn Suppresses NLRP3 Inflammasome Activation


ABSTRACT: The NLRP3 inflammasome is a multi-protein complex that triggers the activation of the inflammatory protein caspase-1 and the maturation of the cytokine IL-1 in response to microbes and other danger signals in host cells. Here, we sought a deeper understanding of how the NLRP3 inflammasome is regulated. We found that inflammasome activation induced the Src family kinase Lyn to phosphorylate NLRP3 at Tyr918, and that this phosphorylation of NLRP3 correlated with a subsequent increase in its ubiquitination, which facilitated its proteasome-mediated degradation. NLRP3 tyrosine phosphorylation and ubiquitination was abrogated in Lyn-deficient macrophages, which produced increased amounts of IL-1. Furthermore, mice lacking Lyn were highly susceptible to LPS-induced septic shock in an NLRP3-dependent manner. Our data demonstrate that Lyn-mediated tyrosine phosphorylation of NLRP3 is a prerequisite for its ubiquitination, thus dampening NLRP3 inflammasome activity.

INSTRUMENT(S): Q Exactive HF

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Dendritic Cell, Bone Marrow

DISEASE(S): Wounds And Injuries

SUBMITTER: robert Pope  

LAB HEAD: Jian Zhang, M.D.

PROVIDER: PXD028582 | Pride | 2022-05-19

REPOSITORIES: Pride

Dataset's files

Source:
Action DRS
18ATPandLPS.msf Msf
ATP.raw Raw
ATP.raw_20210915_Byonic.mgf Mgf
ATP.raw_20210915_Byonic.mzid.gz Mzid
LPS.raw Raw
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Publications

Tyrosine phosphorylation of NLRP3 by the Src family kinase Lyn suppresses the activity of the NLRP3 inflammasome.

Tang Juan J   Xiao Yizhi Y   Lin Guoxin G   Guo Hui H   Deng Han-Xiang HX   Tu Sha S   Langdon Wallace Y WY   Yang Huixiang H   Tao Lijian L   Li Yalan Y   Pope R Marshall RM   Gupta Neetu N   Zhang Jian J  

Science signaling 20211026 706


In response to microbes and other danger signals, the NLRP3 inflammasome in immune cells triggers the activation of the protease caspase-1, which mediates the maturation of the inflammatory cytokine IL-1β. Here, we investigated how the NLRP3 inflammasome is regulated. We found that its activation in primary mouse macrophages induced the Src family kinase Lyn to phosphorylate NLRP3 at Tyr<sup>918</sup>, which correlated with a subsequent increase in its ubiquitination that facilitated its proteas  ...[more]

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