Dataset Information

Discovery of kinetochore and spindle assembly checkpoint proteins in apicomplexan parasites

ABSTRACT: Kinetochores are multiprotein assemblies that bind chromosomes to the spindle during mitosis. Despite conserved roles, repertoires of kinetochore proteins vary greatly across eukaryotes. In particular, most known components are not clearly detected in a group of parasites known as the Apicomplexa. Furthermore, flexibility in scale of amplification and an apparent incapability to delay cell cycle progression in response to spindle integrity has coined an idea these parasites divide in the absence of spindle checkpoints. In this study, we reunite divergent apicomplexan kinetochore components to a common eukaryotic set and additionally identify 9 kinetochore proteins that share little homology to known proteins. AKiTs are essential for chromosome segregation and show modes of division parallel to eukaryotic metaphase to anaphase transition and spindle assembly checkpoint signaling. These findings suggest conserved spindle assembly checkpoint signaling maintains fidelity during chromosome segregation in apicomplexan parasites.

INSTRUMENT(S): Q Exactive HF

ORGANISM(S): Plasmodium Berghei Anka

SUBMITTER: alexandre Hainard

LAB HEAD: Mathieu Brochet

PROVIDER: PXD028595 | Pride | 2023-03-11

REPOSITORIES: Pride

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			Action	DRS
	20191031_QEHF25CM30_pl19099_LBrusini_F2.raw	Raw
	20191031_QEHF25CM30_pl19099_LBrusini_F2__F011197_.mzid.gz	Mzid
	20191031_QEHF25CM30_pl19099_LBrusini_F2__F011197_.mzid_20191031_QEHF25CM30_pl19099_LBrusini_F2__F011197_.MGF	Mzid
	20191031_QEHF25CM32_pl19099_LBrusini_X.raw	Raw
	20191031_QEHF25CM32_pl19099_LBrusini_X__F011198_.mzid.gz	Mzid

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Publications

Composition and organization of kinetochores show plasticity in apicomplexan chromosome segregation.

Brusini Lorenzo L Dos Santos Pacheco Nicolas N Tromer Eelco C EC Soldati-Favre Dominique D Brochet Mathieu M

The Journal of cell biology 20220825 9

Kinetochores are multiprotein assemblies directing mitotic spindle attachment and chromosome segregation. In apicomplexan parasites, most known kinetochore components and associated regulators are apparently missing, suggesting a minimal structure with limited control over chromosome segregation. In this study, we use interactomics combined with deep homology searches to identify 13 previously unknown components of kinetochores in Apicomplexa. Apicomplexan kinetochores are highly divergent in se ...[more]

PMID: 36006241

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Project description:Ibrahim2008 - Mitotic Spindle Assembly Checkpoint - Convey variant The Mitotic Spindle Assembly Checkpoint ((M)SAC) is an evolutionary conserved mechanism. This model incorporates the perspectives of three central control pathways, namely Mad1/Mad2 induced Cdc20 sequestering based on the Template Model, MCC formation, and APC inhibition. MCC:APC dissociation is described by two alternatives models, namely the "Dissociation" and the "Convey" model variants. Both these model are available in BioModels Database. This model corresponds to the "Convey" variant. This model is described in the article: In-silico modeling of the mitotic spindle assembly checkpoint. Ibrahim B, Diekmann S, Schmitt E, Dittrich P PLoS One. 2008 Feb 6;3(2):e1555. Abstract: BACKGROUND: The Mitotic Spindle Assembly Checkpoint ((M)SAC) is an evolutionary conserved mechanism that ensures the correct segregation of chromosomes by restraining cell cycle progression from entering anaphase until all chromosomes have made proper bipolar attachments to the mitotic spindle. Its malfunction can lead to cancer. PRINCIPLE FINDINGS: We have constructed and validated for the human (M)SAC mechanism an in silico dynamical model, integrating 11 proteins and complexes. The model incorporates the perspectives of three central control pathways, namely Mad1/Mad2 induced Cdc20 sequestering based on the Template Model, MCC formation, and APC inhibition. Originating from the biochemical reactions for the underlying molecular processes, non-linear ordinary differential equations for the concentrations of 11 proteins and complexes of the (M)SAC are derived. Most of the kinetic constants are taken from literature, the remaining four unknown parameters are derived by an evolutionary optimization procedure for an objective function describing the dynamics of the APC:Cdc20 complex. MCC:APC dissociation is described by two alternatives, namely the "Dissociation" and the "Convey" model variants. The attachment of the kinetochore to microtubuli is simulated by a switching parameter silencing those reactions which are stopped by the attachment. For both, the Dissociation and the Convey variants, we compare two different scenarios concerning the microtubule attachment dependent control of the dissociation reaction. Our model is validated by simulation of ten perturbation experiments. CONCLUSION: Only in the controlled case, our models show (M)SAC behaviour at meta- to anaphase transition in agreement with experimental observations. Our simulations revealed that for (M)SAC activation, Cdc20 is not fully sequestered; instead APC is inhibited by MCC binding. This model describes the controlled dissociation variant of the mitotic spindle assembly checkpoint. If the tool you use has problems with events, you can uncomment the assignment rules for u and u_prime and comment out the list of events. In accordance with the authors due to typos in the original publication some initial conditions and parameters were slightly changed in the model: article model [O-Mad2] 1.5e-7 M 1.3e-7 M [BubR1:Bub3] 1.30e-7 M 1.27e-7 M k -4 0.01 M -1 s -1 0.02 M -1 s -1 k -5 0.1 M -1 s -1 0.2 M -1 s -1 This model is hosted on BioModels Database and identified by: MODEL6655578762 . To cite BioModels Database, please use: BioModels Database: An enhanced, curated and annotated resource for published quantitative kinetic models . To the extent possible under law, all copyright and related or neighbouring rights to this encoded model have been dedicated to the public domain worldwide. Please refer to CC0 Public Domain Dedication for more information.

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