Proteomics

Dataset Information

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Proteome of extracellular vesicles from the filamentous fungal pathogen Fusarium graminearum


ABSTRACT: We performed a comparative study to determine the proteome of extracellular vesicles (EVs) from the cereal pathogen Fusarium graminearum (Fgr), recovered from in vitro cultures. Label-free quantitative proteomics was used to find significant enrichment of proteins between EV samples, the secretome (secreted-soluble proteins) and the cell lysate. Our results show that some proteins were exclusive to EVs and were upregulated compared to the secretome or cell lysate.

INSTRUMENT(S): Q Exactive HF

ORGANISM(S): Fusarium Graminearum

TISSUE(S): Hyphal Cell, Fungal Cell

SUBMITTER: Donovan Garcia Ceron  

LAB HEAD: Marilyn Anne Anderson

PROVIDER: PXD028657 | Pride | 2022-02-16

REPOSITORIES: Pride

Dataset's files

Source:
Action DRS
EV1_Fgr.raw Raw
EV1_Fgr_20191008223115.raw Raw
EV2_Fgr.raw Raw
EV2_Fgr_20191009072256.raw Raw
EV3_Fgr.raw Raw
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Publications

Extracellular Vesicles from <i>Fusarium</i> <i>graminearum</i> Contain Protein Effectors Expressed during Infection of Corn.

Garcia-Ceron Donovan D   Lowe Rohan G T RGT   McKenna James A JA   Brain Linda M LM   Dawson Charlotte S CS   Clark Bethany B   Berkowitz Oliver O   Faou Pierre P   Whelan James J   Bleackley Mark R MR   Anderson Marilyn A MA  

Journal of fungi (Basel, Switzerland) 20211117 11


<i>Fusarium</i>&nbsp;<i>graminearum</i> (<i>Fgr</i>) is a devastating filamentous fungal pathogen that causes diseases in cereals, while producing mycotoxins that are toxic for humans and animals, and render grains unusable. Low efficiency in managing <i>Fgr</i> poses a constant need for identifying novel control mechanisms. Evidence that fungal extracellular vesicles (EVs) from pathogenic yeast have a role in human disease led us to question whether this is also true for fungal plant pathogens.  ...[more]

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