Dataset Information

Structural insights into the catalytic cycle of a bacterial multidrug ABC efflux pump

ABSTRACT: ABC (“ATP-Binding Cassette”) exporters of the type IV subfamily gather transporters involved in the efflux of many compounds and notably those capable to confer multidrug resistance like the mammalian P-glycoprotein or the bacterial transporter BmrA. They function according to an alternating access mechanism between inward-facing (IF) and outward-facing (OF) conformations, but the extent of physical separation between the two nucleotide-binding domains (NBDs) in different states is still unsettled. Small Angle Neutron Scattering (SANS) and hydrogen/deuterium exchange coupled to mass spectrometry (HDX-MS) were used to highlight different conformational states of BmrA during its catalytic cycle. In particular, mutation of the invariant Lysine residue of the Walker-A motif (K380A) captures BmrA in an ATP-bound IF conformation prior to NBD closure. While in the transition-like state induced by vanadate, wild-type BmrA is mainly in an OF conformation, it and populates only IF conformations only in the presence of ADP/Mg. Importantly, in this post-hydrolytic step, distances between the two NBDs of BmrA appears to be more separated than in the apo state, but they remains shorter than in the widest opening found in the related MsbA transporter. Overall, our results highlight the main steps of the catalytic cycle of a homodimeric bacterial multidrug transporter and underline structural and functional commonalities as well as oddities among the type IV subfamily of ABC exporters.

INSTRUMENT(S): Synapt MS

ORGANISM(S): Bacillus Subtilis

SUBMITTER: Julien Marcoux

LAB HEAD: Julien Marcoux

PROVIDER: PXD028663 | Pride | 2022-03-24

REPOSITORIES: Pride

ACCESS DATA

Dataset's files

Source:

			Action	DRS
	BmrA-K380A-His-Pepsin.fasta	Fasta
	BmrA-WT-His-Pepsin.fasta	Fasta
	K380A_BmrA_LMNG-Clusterfile.csv	Csv
	K380A_BmrA_LMNG-Statefile.csv	Csv
	K380A_BmrA_LMNG_ATPMg.pml	Other

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Publications

Structural Insights into the Catalytic Cycle of a Bacterial Multidrug ABC Efflux Pump.

Javed Waqas W Vallet Sylvain S Clement Marie-Pierre MP Le Roy Aline A Moulin Martine M Härtlein Michael M Breyton Cécile C Burlet-Schiltz Odile O Marcoux Julien J Orelle Cédric C Ebel Christine C Martel Anne A Jault Jean-Michel JM

Journal of molecular biology 20220312 9

ABC ("ATP-Binding Cassette") transporters of the type IV subfamily consist of exporters involved in the efflux of many compounds, notably those capable to confer multidrug resistance like the mammalian P-glycoprotein or the bacterial transporter BmrA. They function according to an alternating access mechanism between inward-facing (IF) and outward-facing (OF) conformations, but the extent of physical separation between the two nucleotide-binding domains (NBDs) in different states is still unsett ...[more]

PMID: 35292347

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