Dataset Information

Five Wheat Species Flour Proteomics by LC-MS/MS

ABSTRACT: LC-MS/MS-based LFQ proteomics of 150 wheat full-kernel flours from five different wheat species (common wheat, spelt, durum wheat, emmer, einkorn) grown at three different locations.

INSTRUMENT(S): Synapt MS

ORGANISM(S): Triticum Aestivum (wheat)

SUBMITTER: Malte Sielaff

LAB HEAD: Stefan Tenzer

PROVIDER: PXD028676 | Pride | 2023-03-30

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	2020-026_Longin_TeslaT_user_designed_20210210-170446_peptide_quantification_report.csv	Csv
	2020-026_Longin_TeslaT_user_designed_20210210-170446_quantification_report.xlsx	Xlsx
	2020-026_sample_info.xlsx	Xlsx
	20201019_UniProtKB202005_4564Triticum_367831entries.fasta	Fasta
	20201019_UniProtKB202005_4564Triticum_367831entries_contaminated_reversed_pipelineFriendly.fasta	Fasta

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Publications

Reference proteomes of five wheat species as starting point for future design of cultivars with lower allergenic potential.

Afzal Muhammad M Sielaff Malte M Distler Ute U Schuppan Detlef D Tenzer Stefan S Longin C Friedrich H CFH

NPJ science of food 20230325 1

Wheat is an important staple food and its processing quality is largely driven by proteins. However, there is a sizable number of people with inflammatory reactions to wheat proteins, namely celiac disease, wheat allergy and the syndrome of non-celiac wheat sensitivity. Thus, proteome profiles should be of high importance for stakeholders along the wheat supply chain. We applied liquid chromatography-tandem mass spectrometry-based proteomics to establish the flour reference proteome for five whe ...[more]

PMID: 36966156

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Project description:Background: MicroRNAs are endogenous small noncoding RNAs that play critical roles in plant abiotic stress responses. The interaction between miRNA-mRNA targets and their regulatory pathways in response to water deficit stress has been investigated in many plant species. However, the miRNA transcriptome of durum wheat (Triticum turgidum L. ssp. durum) is poorly characterised, with little known about miRNA functions related to water deficit stress. Yield loss in durum wheat can be exacerbated due to minimal rainfall in the early reproductive stages of development during Spring in Australia. This study describes genotypic differences in the miRNAome between water deficit tolerant/sensitive durum, using flag leaf and developing head tissue, and more specifically identifies miRNAs associated with water deficit stress. Results: Small RNA libraries (96 in total) were constructed from flag leaf and developing head tissues of four durum genotypes (Tamaroi, Yawa, EGA Bellaroi, Tjilkuri), with or without water deficit stress. Illumina sequencing and subsequent analysis detected 110 conserved miRNAs and 159 novel candidate miRNA hairpins. Statistical analysis of the abundance of sequencing reads revealed 66 conserved miRNAs and five novel miRNA hairpins showing differential expression under water deficit stress. During stress, several conserved and novel miRNAs showed unambiguous inverted regulatory profiles between the durum genotypes studied. Several miRNAs were also identified to have different abundance in the flag leaf compared to the developing head regardless of treatment. Predicted mRNA targets from four novel durum miRNAs were characterised using Gene Ontology (GO) which revealed functions common to stress responses and plant development. Conclusion: For the first time, we present a comprehensive study of the miRNA transcriptome of flag leaf and developing head tissues in different durum genotypes under water deficit stress. The identification of differentially expressed miRNAs provides molecular evidence that miRNAs are potential determinants of water stress tolerance in durum wheat. GO analysis of predicted targets contributes to the understanding of genotype-specific physiological responses leading to stress tolerance capacity. Further functional analysis of specific stress responsive miRNAs identified, and their interaction with mRNA targets is ongoing and will assist in developing future durum wheat varieties with enhanced water deficit stress tolerance.