Dataset Information

Proteomics characterization of evolved Lactococcus lactis mutants obtained from laboratory evolution showed elevated vitamin K2 content and enhanced resistance to oxidative stress

ABSTRACT: This study aimed to obtain Lactococcus lactis strains with high vitamin K2 content via a non-GM approach, i.e., adaptive laboratory evolution, and to characterize the evolved strains.

INSTRUMENT(S): Q Exactive HF-X

ORGANISM(S): Lactococcus Lactis Subsp. Cremoris Mg1363

SUBMITTER: Sjef Boeren

LAB HEAD: Sjef Boeren

PROVIDER: PXD028721 | Pride | 2021-11-02

REPOSITORIES: Pride

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Lactococcus lactis Mutants Obtained From Laboratory Evolution Showed Elevated Vitamin K2 Content and Enhanced Resistance to Oxidative Stress.

Liu Yue Y de Groot Anteun A Boeren Sjef S Abee Tjakko T Smid Eddy J EJ

Frontiers in microbiology 20211014

Vitamin K2 is an important vitamin for human health. Vitamin K2 enrichment in the human diet is possible by using vitamin K2-producing bacteria such as Lactococcus lactis in food fermentations. Based on previous observations that aerated cultivation conditions improved vitamin K2 content in L. lactis, we performed laboratory evolution on L. lactis MG1363 by cultivating this strain in a shake flask in a sequential propagation regime with transfers to a fresh medium every 72h. ...[more]

PMID: 34721346

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Project description:Amino acid assimilation and metabolism are crucial for bacterial growth and survival and this is particularly obvious for lactic acid bacteria (LAB) that are generally auxotroph for various amino acids. However, amino acid assimilation is poorly characterized and a complete description of the response during amino acid starvation is still lacking in LAB. In this context, the global response of the LAB model Lactococcus lactis was characterized during isoleucine starvation in batch culture. The stress was imposed by isoleucine natural consumption in an initially rich chemically defined medium. Dynamic analyses were performed both using transcriptomic and proteomic approaches. The response was found to occur gradually and could be divided into three major parts that were firstly deduced from transcriptomic analysis and generally corroborated by proteomic results: (i) a global repression of biogenic processes (transcription, translation, and carbon metabolism and transport), (ii) a specific response related to the limiting nutrient (numerous pathways belonging to carbon or nitrogen metabolism and leading to isoleucine supply were activated) and (iii) an additional response connected to oxidative stress (induction of aerobic metabolism, electron transport, thioredoxin metabolism and pyruvate dehydrogenase). The involvement of various regulatory mechanisms such as growth rate regulation, stringent response, CodY, GlnR, and CcpA regulations, was discussed on the basis of transcriptomic data comparisons. Above the full description of L. lactis isoleucine starvation response, this work additionally provided a complex but realistic outlook of the regulation network involved in isoleucine starvation. Such integrated and comparative approach will allow, by its implementation to other regulations and environmental conditions, the whole regulatory network of L. lactis or any other microorganism to be deciphered. Batch cultivation of Lactococcus lactis IL1403 were carried out on a chemically defined medium and under controlled conditions (30 °C, pH 6.6, nitrogen atmosphere). Cell samples were harvested at steady state. Total RNA was extracted from these samples and radiolabelled cDNA were prepared and hybridized on nylon arrays. 1948 amplicons specific of Lactococcus lactis IL1403 genes were spotted twice on the array. Samples corresponding to various growth rates were analyzed simultaneously and 3 independent repetitions were performed.

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	MQresult_TA232-267.zip	Other
	TA232_1.raw	Raw
	TA233_2.raw	Raw
	TA234_3.raw	Raw
	TA235_4.raw	Raw

Dataset Information

Proteomics characterization of evolved Lactococcus lactis mutants obtained from laboratory evolution showed elevated vitamin K2 content and enhanced resistance to oxidative stress

Dataset's files

Publications

<i>Lactococcus lactis</i> Mutants Obtained From Laboratory Evolution Showed Elevated Vitamin K2 Content and Enhanced Resistance to Oxidative Stress.

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