Dataset Information

Organoid differentiation with IL22

ABSTRACT: Differentiation of human organoids with IL22, and/or the inhibition of the same process with STAT3 or mTOR inhibitors.

INSTRUMENT(S): Orbitrap Exploris 480

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Epithelial Cell, Cell Culture

DISEASE(S): Disease Free

SUBMITTER: wei wu

LAB HEAD: Wei Wu

PROVIDER: PXD028746 | Pride | 2022-10-13

REPOSITORIES: Pride

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Dataset's files

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			Action	DRS
	20210831_EX2_UM1_wu000024_SA_EXT02_GW_1_R1_F1.raw	Raw
	20210831_EX2_UM1_wu000024_SA_EXT02_GW_1_R1_F2.raw	Raw
	20210831_EX2_UM1_wu000024_SA_EXT02_GW_1_R1_F3.raw	Raw
	20210831_EX2_UM1_wu000024_SA_EXT02_GW_1_R1_F4.raw	Raw
	20210831_EX2_UM1_wu000024_SA_EXT02_GW_1_R1_F5.raw	Raw

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Publications

Optimized human intestinal organoid model reveals interleukin-22-dependency of paneth cell formation.

He Gui-Wei GW Lin Lin L DeMartino Jeff J Zheng Xuan X Staliarova Nadzeya N Dayton Talya T Begthel Harry H van de Wetering Willine J WJ Bodewes Eduard E van Zon Jeroen J Tans Sander S Lopez-Iglesias Carmen C Peters Peter J PJ Wu Wei W Kotlarz Daniel D Klein Christoph C Margaritis Thanasis T Holstege Frank F Clevers Hans H

Cell stem cell 20220823 9

Opposing roles have been proposed for IL-22 in intestinal pathophysiology. We have optimized human small intestinal organoid (hSIO) culturing, constitutively generating all differentiated cell types while maintaining an active stem cell compartment. IL-22 does not promote the expansion of stem cells but rather slows the growth of hSIOs. In hSIOs, IL-22 is required for formation of Paneth cells, the prime producers of intestinal antimicrobial peptides (AMPs). Introduction of inflammatory bowel di ...[more]

PMID: 36002022

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