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N-terminome analyses of the intramembrane-cleaving protease SPPL3

ABSTRACT: The intramembrane protease SPPL3 can specifically cleave select Golgi enzymes, enabling their secretion and concomitantly altering global cellular glycosylation, yet the entire range of Golgi glycan-modifying enzymes cleaved by SPPL3 under physiological conditions remains to be defined. Here, we established isogenic SPPL3-deficient HEK293 and HeLa cell lines and applied N-terminomics to identify substrates cleaved by endogenous SPPL3 and released into conditioned cell culture supernatants.

INSTRUMENT(S): Orbitrap Fusion Lumos, Q Exactive HF, Q Exactive

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Cell Culture

SUBMITTER: Andreas Tholey

LAB HEAD: Andreas Tholey

PROVIDER: PXD028769 | Pride | 2022-03-17

REPOSITORIES: Pride

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			Action	DRS
	HEK_SN_HYTANE_45_60_75.raw	Raw
	HEK_SN_HYTANE_50_65_85.raw	Raw
	HEK_SN_HYTANE_FAIMS.pdResult	Other
	LB_LF_Golgi_separate_try_semiTry.pdResult	Other
	LB_LF_KO_1.raw	Raw

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